Enzymes Recombinant Proteins

Human PMVK Recombinant Protein (RPPB2623)

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SKU:
RPPB2623
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q15126
Research Area:
Enzymes

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human PMVK Recombinant Protein
Product Code:RPPB2623
Size:20µg
Species:Human
Target:PMVK
Synonyms:Phosphomevalonate kinase, PMKase, hPMK, PMVK, PMKI, PMK, PMKA, PMKASE, HUMPMKI.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The PMVK solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 7.5), 1mM DTT, 10% glycerol and 0.1M NaCl.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MAPLGGAPRL VLLFSGKRKS GKDFVTEALQ SRLGADVCAV LRLSGPLKEQ YAQEHGLNFQ RLLDTSTYKE AFRKDMIRWG EEKRQADPGF FCRKIVEGIS QPIWLVSDTR RVSDIQWFRE AYGAVTQTVR VVALEQSRQQ RGWVFTPGVD DAESECGLDN FGDFDWVIEN HGVEQRLEEQ LENLIEFIRS RL

Phosphomevalonate kinase (PMVK) is a cytosolic enzyme. PMVK is expressed highly in the heart, liver, skeletal muscle, kidney and pancreas and slightly lower in the brain, placenta, and lung. PMVK catalyzes the conversion of mevalonate 5-phosphate into mevalonate 5-diphosphate in the fifth reaction of the cholesterol biosynthetic pathway. Induced by sterol, PMVK participates in isopentenyl diphosphate biosynthesis via the mevalonate pathway.

PMVK Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 212 amino acids (1-192 a.a.) and having a molecular mass of 24.1kDa. The PMVK is purified by proprietary chromatographic techniques.

UniProt Protein Function:PMVK: Induced by sterol. Monomer.
UniProt Protein Details:

Protein type:Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; EC 2.7.4.2; Kinase, other

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: membrane; peroxisome; cytosol

Molecular Function:phosphomevalonate kinase activity; ATP binding

Biological Process: isopentenyl diphosphate biosynthetic process, mevalonate pathway; sterol biosynthetic process; phosphorylation; cholesterol biosynthetic process

NCBI Summary:This gene encodes a peroxisomal enzyme that catalyzes the conversion of mevalonate 5-phosphate into mevalonate 5-diphosphate, the fifth reaction of the cholesterol biosynthetic pathway. Studies in rat show that the message level and the enzyme activity of this protein is regulated by sterol, and that this regulation is coordinated with 3-hydroxy-3-methylglutaryl coenzyme A reductase, the rate-limiting enzyme of cholesterol biosynthesis. [provided by RefSeq, Sep 2011]
UniProt Code:Q15126
NCBI GenInfo Identifier:3024422
NCBI Gene ID:10654
NCBI Accession:Q15126.3
UniProt Secondary Accession:Q15126,Q5TZW9,
UniProt Related Accession:Q15126
Molecular Weight:21,995 Da
NCBI Full Name:Phosphomevalonate kinase
NCBI Synonym Full Names:phosphomevalonate kinase
NCBI Official Symbol:PMVK��
NCBI Official Synonym Symbols:PMK; PMKA; PMKASE; HUMPMKI��
NCBI Protein Information:phosphomevalonate kinase; hPMK
UniProt Protein Name:Phosphomevalonate kinase
UniProt Gene Name:PMVK��
UniProt Entry Name:PMVK_HUMAN
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system_update_altDatasheetPMVK Recombinant Protein Phosphomevalonate kinase (PMVK) is a cytosolic enzyme. PMVK can be highly expressed in the heart,skeletal muscle, liver, pancreas, and kidney; i