Description
| Product Name: | Human PMM1 Recombinant Protein |
| Product Code: | RPPB2127 |
| Size: | 10µg |
| Species: | Human |
| Target: | PMM1 |
| Synonyms: | Phosphomannomutase 1, PMM 1, PMMH-22, PMM1, PMMH22, Sec53. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The PMM1 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol, 2mM DTT, 100mM NaCl and 0.1mM PMSF. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MAVTAQAARR KERVLCLFDV DGTLTPARQK IDPEVAAFLQ KLRSRVQIGV VGGSDYCKIA EQLGDGDEVI EKFDYVFAEN GTVQYKHGRL LSKQTIQNHL GEELLQDLIN FCLSYMALLR LPKKRGTFIE FRNGMLNISP IGRSCTLEER IEFSELDKKE KIREKFVEAL KTEFAGKGLR FSRGGMISFD VFPEGWDKRY CLDSLDQDSF DTIHFFGNET SPGGNDFEIF ADPRTVGHSV VSPQDTVQRC REIFFPETAH EA |
Phosphomannomutase 1 (PMM1) is an enzyme involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. PMM1 catalyzes the conversion between D-mannose 6-phosphate and D-mannose 1-phosphate which is a substrate for GDP-mannose synthesis. GDP-mannose is used for the synthesis of dolichol-phosphate-mannose, which is crucial for N-linked glycosylation and accordingly the secretion of several glycoproteins as well as for the synthesis of glycosyl-phosphatidyl-inositol (GPI) anchored proteins. Additionally, PMM1 may be responsible for the degradation of glucose-1,6-bisphosphate in ischemic brain.
PMM1 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 282 amino acids (1-262 a.a.) and having a molecular mass of 31.9kDa. The PMM1 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PMM1: Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. In addition, may be responsible for the degradation of glucose-1,6-bisphosphate in ischemic brain. Belongs to the eukaryotic PMM family.Protein type: Carbohydrate Metabolism - amino sugar and nucleotide sugar; Carbohydrate Metabolism - fructose and mannose; EC 5.4.2.8; IsomeraseChromosomal Location of Human Ortholog: 22q13.2Cellular Component: cytosolMolecular Function: phosphomannomutase activity; protein bindingBiological Process: GDP-mannose biosynthetic process; mannose metabolic process; protein amino acid N-linked glycosylation; protein targeting to ER |
| UniProt Protein Details: | |
| NCBI Summary: | Phosphomannomutase catalyzes the conversion between D-mannose 6-phosphate and D-mannose 1-phosphate which is a substrate for GDP-mannose synthesis. GDP-mannose is used for synthesis of dolichol-phosphate-mannose, which is essential for N-linked glycosylation and thus the secretion of several glycoproteins as well as for the synthesis of glycosyl-phosphatidyl-inositol (GPI) anchored proteins. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q92871 |
| NCBI GenInfo Identifier: | 194097356 |
| NCBI Gene ID: | 5372 |
| NCBI Accession: | NP_002667.2 |
| UniProt Secondary Accession: | Q92871,Q92586, A8K003 |
| UniProt Related Accession: | Q92871 |
| Molecular Weight: | 29,747 Da |
| NCBI Full Name: | phosphomannomutase 1 |
| NCBI Synonym Full Names: | phosphomannomutase 1 |
| NCBI Official Symbol: | PMM1�� |
| NCBI Official Synonym Symbols: | Sec53�� |
| NCBI Protein Information: | phosphomannomutase 1 |
| UniProt Protein Name: | Phosphomannomutase 1 |
| UniProt Synonym Protein Names: | PMMH-22 |
| Protein Family: | |
| UniProt Gene Name: | PMM1�� |
| UniProt Entry Name: | PMM1_HUMAN |
