Neurotrophins Recombinant Proteins

Human Pleiotrophin Recombinant Protein (RPPB0854)

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SKU:
RPPB0854
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P21246
Research Area:
Neurotrophins

Description

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Product Name:Human Pleiotrophin Recombinant Protein
Product Code:RPPB0854
Size:20µg
Species:Human
Target:Pleiotrophin
Synonyms:PTN, Heparin Affin Regulatory Protein, HARP, Heparin-binding growth factor-8, HBGF-8, Osteoblast-Specific Factor-1, OSF-1, Heparin-binding growth-associated molecule, HB-GAM, HBNF-1 Heparin-binding brain mitogen, Heparin-binding neurite outgrowth-promoting factor 1, HBBM, NEGF1.
Source:Escherichia Coli
Physical Appearance:Filtered White lyophilized (freeze-dried) powder.
Formulation:Filtered and lyophilized from 0.5 mg/ml in 0.1M phosphate buffer and 0.1M NaCl, pH 7.2.
Solubility:Add 0.2 ml of PBS pH 7.2 and let the lyophilized pellet dissolve completely.
Stability:Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Purity:Greater than 95% as determined by SDS-PAGE.
Amino Acid Sequence:MKHHHHHHHM LVPRGSGKKE KPEKKVKKSD CGEWQWSVCV PTSGDCGLGT REGTRTGAEC KQTMKTQRCK IPCNWKKQFG AECKYQFQAW GECDLNTALK TRTGSLKRAL HNAECQKTVT ISKPCGKLTK PKPQAESKKK KKEGKKQEKM LD

Pleiotrophin (Osteoblast-Specific Factor-1, OSF-1) contains 136 amino acid residues. The sequence is very rich in cationic amino acids (24% of the residues); lysine cluster sequences are found in the N-terminal and C-terminal ends of the structure.The OSF-1 gene was shown by Northern blotting analysis to be expressed in mouse calvarial osteoblast-enriched cells and in mouse brain tissues, but not in thymus, spleen, kidney, liver, lung, testis or heart. Pleiotrophin has the ability to promote adhesion, migration, expansion, and differentiation of human osteoprogenitor cells. In addition to certain types of cancer, the embryonic growth and differentiation factor pleiotrophin is found also in adults in inflammatory diseases. In osteoarthritis, pleiotrophin is especially expressed in early stages, and its concentrations in the synovial fluid could serve as a marker for the progress of the disease. Pleitrophin might be involved in cartilage repair in osteoarthritis, in particular, in earlier stages.

Pleiotrophin Human Recombinant contains His-Tagged Fusion Protein, produced in E. coli, its molecular weight is 17.3 kDa protein containing 136 amino acid residues of the OSF-1 human and 16 additional amino acid residues - HisTag, thrombin cleavage site (underlined).

UniProt Protein Function:PTN: Secreted growth factor that induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells. Binds anaplastic lymphoma kinase (ALK) which induces MAPK pathway activation, an important step in the anti- apoptotic signaling of PTN and regulation of cell proliferation. Belongs to the pleiotrophin family.
UniProt Protein Details:

Protein type:Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7q33

Cellular Component: extracellular space; endoplasmic reticulum

Molecular Function:heparin binding; growth factor activity; protein phosphatase inhibitor activity

Biological Process: nervous system development; positive regulation of cell division; negative regulation of catalytic activity; positive regulation of cell proliferation; learning; transmembrane receptor protein tyrosine phosphatase signaling pathway; bone mineralization

UniProt Code:P21246
NCBI GenInfo Identifier:131553
NCBI Gene ID:5764
NCBI Accession:P21246.1
UniProt Secondary Accession:P21246,Q5U0B0, Q6ICQ5, Q9UCC6,
UniProt Related Accession:P21246
Molecular Weight:18,942 Da
NCBI Full Name:Pleiotrophin
NCBI Synonym Full Names:pleiotrophin
NCBI Official Symbol:PTN��
NCBI Official Synonym Symbols:HARP; HBNF; HBGF8; NEGF1��
NCBI Protein Information:pleiotrophin; HBBM; OSF-1; HB-GAM; HBGF-8; HBNF-1; osteoblast-specific factor 1; heparin-binding brain mitogen; heparin binding growth factor 8; heparin-binding growth factor 8; heparin affin regulatory protein; neurite growth-promoting factor 1; heparin-binding growth-associated molecule; heparin-binding neurite outgrowth-promoting factor 1; pleiotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1)
UniProt Protein Name:Pleiotrophin
UniProt Synonym Protein Names:Heparin-binding brain mitogen; HBBM; Heparin-binding growth factor 8; HBGF-8; Heparin-binding growth-associated molecule; HB-GAM; Heparin-binding neurite outgrowth-promoting factor 1; HBNF-1; Osteoblast-specific factor 1; OSF-1
Protein Family:Pleiotrophin
UniProt Gene Name:PTN��
UniProt Entry Name:PTN_HUMAN
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