Enzymes Recombinant Proteins

Human PLA1A Recombinant Protein (RPPB2117)

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SKU:
RPPB2117
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q53H76
Research Area:
Enzymes

Description

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Product Name:Human PLA1A Recombinant Protein
Product Code:RPPB2117
Size:25µg
Species:Human
Target:PLA1A
Synonyms:Phospholipase A1 Member A, PSPLA1, PS-PLA1, Phosphatidylserine-Specific Phospholipase A1alpha, EC 3.1.1.-, NMD, Phosphatidylserine-Specific Phospholipase A1, PLA1A.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:The PLA1A solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 80% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSDAPPTPQ PKCADFQSAN LFEGTDLKVQ FLLFVPSNPS CGQLVEGSSD LQNSGFNATL GTKLIIHGFR VLGTKPSWID TFIRTLLRAT NANVIAVDWI YGSTGVYFSA VKNVIKLSLE ISLFLNKLLV LGVSESSIHI IGVSLGAHVG GMVGQLFGGQ LGQITGLDPA GPEYTRASVE ERLDAGDALF VEAIHTDTDN LGIRIPVGHV DYFVNGGQDQ PGCPTFFYAG YSYLICDHMR AVHLYISALE NSCPLMAFPC ASYKAFLAGR CLDCFNPFLL SCPRIGLVEQ GGVKIEPLPK EVKVYLLTTS SAPYCMHHSL VEFHLKELRN KDTNIEVTFL SSNITSSSKI TIPKQQRYGK GIIAHATPQC QINQVKFKFQ SSNRVWKKDR TTIIGKFCTA LLPVNDREKM VCLPEPVNLQ ASVTVSCDLK IACV

Phospholipase A1 Member A (PLA1A) is a phospholipase which hydrolyzes fatty acids at the sn-1 position of phosphatidylserine and 1-acyl-2-lysophosphatidylserine. The secreted PLA1A protein hydrolyzes phosphatidylserine in liposomes. PLA1A hydrolyzes phosphatidylserine (PS) in the form of liposomes and 1-acyl-2 lysophosphatidylserine (lyso-PS), but not triolein, phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA) or phosphatidylinositol (PI). PLA1A isoform 2 hydrolyzes lyso-PS but not PS. The hydrolysis of lyso-PS in peritoneal mast cells activated by receptors for IgE leads to stimulation of histamine production.

PLA1A Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 454 amino acids (26-456) and having a molecular mass of 49.5kDa.PLA1A is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:PLA1A: Hydrolyzes the ester bond at the sn-1 position of glycerophospholipids and produces 2-acyl lysophospholipids. Hydrolyzes phosphatidylserine (PS) in the form of liposomes and 1- acyl-2 lysophosphatidylserine (lyso-PS), but not triolein, phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA) or phosphatidylinositol (PI). Isoform 2 hydrolyzes lyso-PS but not PS. Hydrolysis of lyso-PS in peritoneal mast cells activated by receptors for IgE leads to stimulate histamine production. Belongs to the AB hydrolase superfamily. Lipase family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Secreted, signal peptide; EC 3.1.1.-; Secreted; Phospholipase

Chromosomal Location of Human Ortholog: 3q13.13-q13.2

Cellular Component: acrosomal membrane

Molecular Function:phospholipase A1 activity

Biological Process: lipid catabolic process; lipid metabolic process; phosphatidylserine metabolic process

NCBI Summary:The protein encoded by this gene is a phospholipase that hydrolyzes fatty acids at the sn-1 position of phosphatidylserine and 1-acyl-2-lysophosphatidylserine. This secreted protein hydrolyzes phosphatidylserine in liposomes. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2011]
UniProt Code:Q53H76
NCBI GenInfo Identifier:124015212
NCBI Gene ID:51365
NCBI Accession:Q53H76.2
UniProt Secondary Accession:Q53H76,O95991, Q86WX6, Q9UPD2, B2R8V2, B4DXA2,
UniProt Related Accession:Q53H76
Molecular Weight:31,285 Da
NCBI Full Name:Phospholipase A1 member A
NCBI Synonym Full Names:phospholipase A1 member A
NCBI Official Symbol:PLA1A��
NCBI Official Synonym Symbols:PSPLA1; PS-PLA1��
NCBI Protein Information:phospholipase A1 member A
UniProt Protein Name:Phospholipase A1 member A
UniProt Synonym Protein Names:Phosphatidylserine-specific phospholipase A1; PS-PLA1
Protein Family:Phospholipase
UniProt Gene Name:PLA1A��
UniProt Entry Name:PLA1A_HUMAN
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