Enzymes Recombinant Proteins

Human PINK1 Recombinant Protein (RPPB2610)

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RPPB2610

Description

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Product Name:Human PINK1 Recombinant Protein
Product Code:RPPB2610
Size:20µg
Species:Human
Target:PINK1
Synonyms:PTEN induced putative kinase 1, PARK6, protein kinase BRPK, Parkinson disease (autosomal recessive) 6, serine/threonine-protein kinase PINK1 mitochondrial, EC 2.7.11.1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The PINK1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1M Urea and 5% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MYLIGQSIGK GCSAAVYEAT MPTLPQNLEV TKSTGLLPGR GPGTSAPGEG QERAPGAPAF PLAIKMMWNI SAGSSSEAIL NTMSQELVPA SRVALAGEYG AVTYRKSKRG PKQLAPHPNI IRVLRAFTSS VPLLPGALVD YPDVLPSRLH PEGLGHGRTL FLVMKNYPCT LRQYLCVNTP SPRLAAMMLL QLLEGVDHLV QQGIAHRDLK SDNILVELDP DGCPWLVIAD FGCCLADESI GLQLPFSSWY VDRGGNGCLM APEVSTARPG PRAVIDYSKA DAWAVGAIAY EIFGLVNPFY GQGKAHLESR SYQEAQLPAL PESVPPDVRQ LVRALLQREA SKRPSARVAA NVL

PINK1 is a serine/threonine protein kinase which is found in the mitochondria. PINK1 shields cells from stress-induced mitochondrial dysfunction. Mutations results in one form of autosomal recessive early-onset Parkinson disease.

PINK1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 353 amino acids (156-507) and having a molecular mass of 37.9 kDa.PINK1 is purified by proprietary chromatographic techniques.

UniProt Protein Function:PINK1: Protects against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). It is necessary for PARK2 recruitment to dysfunctional mitochondria to initiate their degradation. Interats with PARK2. Highly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral; Protein kinase, Other; Kinase, protein; Mitochondrial; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Other group; NKF2 family

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: mitochondrion; mitochondrial intermembrane space; cytosol; mitochondrial outer membrane; cytoskeleton; membrane; axon; perinuclear region of cytoplasm; mitochondrial inner membrane; cytoplasm; TORC2 complex; chromatin; nucleus; integral to mitochondrial outer membrane

Molecular Function:protein kinase B binding; protein serine/threonine kinase activity; protein binding; protease activator activity; protease binding; ubiquitin protein ligase binding; magnesium ion binding; kinase activity; ATP binding

Biological Process: ubiquitin-dependent protein catabolic process; negative regulation of JNK cascade; positive regulation of dopamine secretion; positive regulation of translation; regulation of protein ubiquitination; protein ubiquitination; protein amino acid phosphorylation; positive regulation of protein amino acid dephosphorylation; negative regulation of macroautophagy; regulation of mitochondrial membrane potential; positive regulation of ubiquitin-protein ligase activity; regulation of protein complex assembly; response to stress; negative regulation of neuron apoptosis; regulation of hydrogen peroxide metabolic process; mitochondrion organization and biogenesis; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein stabilization; activation of protein kinase B; positive regulation of synaptic transmission, dopaminergic; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein kinase B signaling cascade; peptidyl-serine phosphorylation; mitochondrion degradation; positive regulation of transcription factor activity; response to oxidative stress; positive regulation of protein amino acid phosphorylation; phosphorylation

Disease: Parkinson Disease 6, Autosomal Recessive Early-onset

NCBI Summary:This gene encodes a serine/threonine protein kinase that localizes to mitochondria. It is thought to protect cells from stress-induced mitochondrial dysfunction. Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease. [provided by RefSeq, Jul 2008]
UniProt Code:Q9BXM7
NCBI GenInfo Identifier:14165272
NCBI Gene ID:65018
NCBI Accession:NP_115785.1
UniProt Secondary Accession:Q9BXM7,Q8N6T9, Q8NBU3, Q96DE4,
UniProt Related Accession:Q9BXM7
Molecular Weight:
NCBI Full Name:serine/threonine-protein kinase PINK1, mitochondrial
NCBI Synonym Full Names:PTEN induced putative kinase 1
NCBI Official Symbol:PINK1��
NCBI Official Synonym Symbols:BRPK; PARK6��
NCBI Protein Information:serine/threonine-protein kinase PINK1, mitochondrial; protein kinase BRPK; PTEN-induced putative kinase protein 1
UniProt Protein Name:Serine/threonine-protein kinase PINK1, mitochondrial
UniProt Synonym Protein Names:BRPK; PTEN-induced putative kinase protein 1
Protein Family:Serine/threonine-protein kinase
UniProt Gene Name:PINK1��
UniProt Entry Name:PINK1_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9BXM7
Research Area:
Enzymes
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