Enzymes Recombinant Proteins

Human PHPT1 Recombinant Protein (RPPB2109)

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SKU:
RPPB2109
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9NRX4
Research Area:
Enzymes

Description

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Product Name:Human PHPT1 Recombinant Protein
Product Code:RPPB2109
Size:25µg
Species:Human
Target:PHPT1
Synonyms:PHP14, CGI-202, HSPC141, Phosphohistidine Phosphatase 1, Phosphohistidine Phosphatase 14kDa, Protein janus-A homolog, Sex-regulated protein Janus-a.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:The PHPT1 protein solution (0.5mg/1ml) is formulated in 20 mM Tris-HCl buffer (pH8.0) 0.2M NaCl, 2mM DTT and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MAVADLALIP DVDIDSDGVF KYVLIRVHSA PRSGAPAAES KEIVRGYKWA EYHADIYDKV SGDMQKQGCD CECLGGGRIS HQSQDKKIHV YGYSMAYGPA QHAISTEKIK AKYPDYEVTW ANDGY

PHPT1 is a member of the Janus protein familyand is 125 amino acid long. PHPT1 appears as a monomer in the cytoplasm and is an EDTA-insensitive phosphohistidine phosphatase. Overexpression of PHPT1 resolts in specific phosphohistidine phosphatase activity towards phosphopeptide I, with no activity detected towards phosphotyrosine, phosphothreonine and phosphoserine peptides.

PHPT1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 145 amino acids (1-125a.a.) and having a molecular mass of 15.9kDa.PHPT1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:PHPT1: a phosphohistidine phosphatase. Present preferentially in heart and skeletal muscle. Insensitive to inhibition by okadaic acid and EDTA.
UniProt Protein Details:

Protein type:Carbohydrate Metabolism - fructose and mannose; Phosphatase; Cofactor and Vitamin Metabolism - riboflavin; EC 3.1.3.-; Motility/polarity/chemotaxis; Cofactor and Vitamin Metabolism - thiamine

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: cytosol

Molecular Function:phosphohistidine phosphatase activity; calcium channel inhibitor activity; phosphoprotein phosphatase activity

Biological Process: negative regulation of lyase activity; negative regulation of T cell receptor signaling pathway; protein amino acid dephosphorylation

NCBI Summary:This gene encodes an enzyme that catalyzes the reversible dephosphorylation of histidine residues in proteins. It may be involved in the dephosphorylation of G-beta and ATP citrate lyase and in negatively regulating CD4 T lymphocytes by dephosphorylation and inhibition of KCa3.1 channels. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]
UniProt Code:Q9NRX4
NCBI GenInfo Identifier:25008934
NCBI Gene ID:29085
NCBI Accession:Q9NRX4.1
UniProt Secondary Accession:Q9NRX4,Q9H0Y3, B1AMX0, B1AMX1,
UniProt Related Accession:Q9NRX4
Molecular Weight:125
NCBI Full Name:14 kDa phosphohistidine phosphatase
NCBI Synonym Full Names:phosphohistidine phosphatase 1
NCBI Official Symbol:PHPT1��
NCBI Official Synonym Symbols:PHP14; CGI-202; HSPC141; HEL-S-132P��
NCBI Protein Information:14 kDa phosphohistidine phosphatase; protein janus-A homolog; sex-regulated protein janus-a; epididymis secretory sperm binding protein Li 132P
UniProt Protein Name:14 kDa phosphohistidine phosphatase
UniProt Synonym Protein Names:Phosphohistidine phosphatase 1; Protein janus-A homolog
Protein Family:14 kDa phosphohistidine phosphatase
UniProt Gene Name:PHPT1��
UniProt Entry Name:PHP14_HUMAN
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