Human Phosphorylation Pathway Profiling Array (55 targets) (SARB0063)

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SKU:
SARB0063
Product Type:
Phosphorylation Array
Analytes:
4E-BP1 (P-Thr36)
Analytes:
AKT (P-Ser473)
Analytes:
AMPKa (P-Thr172)
Analytes:
ATF2 (P-Thr69/71)
Analytes:
ATM (P-Ser1981)
Analytes:
BAD (P-Ser112)
Analytes:
C-Fos (P-Thr232)
Analytes:
c-Jun (P-Ser73)
Analytes:
CREB (P-Ser133)
Analytes:
EGFR (P-Ser1070)
Analytes:
eIF-2a (P-Ser51)
Analytes:
ERK1/2 (P-Thr202/P-Tyr204/P-Tyr185/P-Tyr187)
Analytes:
GSK-3 alpha (P-Ser21)
Analytes:
GSK-3 beta (P-Ser9)
Analytes:
HDAC2 (P-Ser394)
Analytes:
HDAC4 (P-Ser632)
Analytes:
HSP27 (P-Ser82)
Analytes:
IKBa (P-Ser32)
Analytes:
JAK1 (P-Tyr1022)
Analytes:
JAK2 (P-Tyr1007/1008)
Analytes:
JNK (P-Thr183)
Analytes:
MEK (P-Ser217/221)
Analytes:
MKK3 (P-Ser189)
Analytes:
MKK6 (P-Ser207) (MEK6)
Analytes:
MSK1 (P-Ser376)
Analytes:
MSK2 (P-Ser360)
Analytes:
mTOR (P-Ser2448)
Analytes:
NF-kB (P-Ser536)
Analytes:
p27 (P-Thr198)
Analytes:
p38 (P-Thr180/Tyr182)
Analytes:
P53 (P-Ser15)
Analytes:
P70S6K (P-Thr421/Ser424)
Analytes:
PDK1 (P-Ser241)
Analytes:
PRAS40 (P-Thr246)
Analytes:
PTEN (P-Ser380)
Analytes:
Raf-1 (P-Ser301)
Analytes:
RPS6 (P-Ser235/236)
Analytes:
RSK1 (P-Ser380)
Analytes:
RSK2 (P-Ser386)
Analytes:
SHP-1 (P-Ser591)
Analytes:
SHP2 (P-Tyr542)
Analytes:
SMAD1 (P-Ser463/465)
Analytes:
SMAD2 (P-Ser245/250/255)
Analytes:
SMAD4 (P-Thr277)
Reactivity:
Human
Applications:
Multiplex Array

Description

system_update_altDatasheet
Product Sku:SARB0063
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:55
Gene Symbols:AKT1, AKT1S1, AMPK1, AND, ATF2, ATM, BAD, CDKN1B, CREB1, EGFR|EIF2S1, EIF4EBP1, FOS, GSK3A, GSK3B, HDAC2, HDAC4, HSPB1, JAK1, JAK2, JUN, MAP2K1, MAP2K3, MAP2K6, MAP3K7, MAPK14, MAPK8, MTOR, NFKBIA, PDPK1, PTEN, PTPN11, PTPN6, RAF1, RELA, RPS6, RPS6KA1, RPS6KA3, RPS6KA4, RPS6KA5, RPS6KB1, SMAD1, SMAD2, SMAD4, SMAD5, SRC, STAT1, STAT2, STAT3, STAT5A, STAT6, TBK1, TP53, TYK2, ZAP70
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:Akt Signaling / JAK/STAT Signaling / MAPK Signaling / NFkB Signaling / TGF-beta Signaling
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
4E-BP1 (P-Thr36)
  • Gene Symbol: EIF4EBP1
  • Gene ID: 1978
  • Accession #: Q13541
AKT (P-Ser473)
  • Gene Symbol: Akt1 Akt Rac
  • Gene ID: 11651
  • Accession #: P31750
AMPKa (P-Thr172)
  • Gene Symbol: AMPK1
  • Gene ID: 5562
  • Accession #: P54645
ATF2 (P-Thr69/71)
  • Gene Symbol: ATF2 CREB2 CREBP1
  • Gene ID: 1386;
  • Accession #: P15336
ATM (P-Ser1981)
  • Gene Symbol: ATM
  • Gene ID: 472;
  • Accession #: Q13315
BAD (P-Ser112)
  • Gene Symbol: Bad Bbc6
  • Gene ID: 12015
  • Accession #: Q61337
C-Fos (P-Thr232)
  • Gene Symbol: FOS G0S7
  • Gene ID: 2353;
  • Accession #: P01100
c-Jun (P-Ser73)
  • Gene Symbol: JUN
  • Gene ID: 3725;
  • Accession #: P05412
CREB (P-Ser133)
  • Gene Symbol: CREB1
  • Gene ID: 1385
  • Accession #: P16220
EGFR (P-Ser1070)
  • Gene Symbol: EGFR ERBB ERBB1 HER1
  • Gene ID: 1956
  • Accession #: P00533
eIF-2a (P-Ser51)
  • Gene Symbol: EIF2S1 EIF2A
  • Gene ID: 1965;
  • Accession #: P05198
ERK1/2 (P-Thr202/P-Tyr204/P-Tyr185/P-Tyr187)
  • Gene Symbol: AND ERK1 ERK2 MAPK1 MAPK3 PRKM1 PRKM2 PRKM3
  • Gene ID: 5595 5594
  • Accession #: P27361 P28482
GSK-3 alpha (P-Ser21)
  • Gene Symbol: GSK3A
  • Gene ID: 2931
  • Accession #: P49840
GSK-3 beta (P-Ser9)
  • Gene Symbol: GSK3B
  • Gene ID: 2932;
  • Accession #: P49841
HDAC2 (P-Ser394)
  • Gene Symbol: HDAC2
  • Gene ID: 3066
  • Accession #: Q92769
HDAC4 (P-Ser632)
  • Gene Symbol: HDAC4 KIAA0288
  • Gene ID: 9759;
  • Accession #: P56524
HSP27 (P-Ser82)
  • Gene Symbol: Hspb1 Hsp25 Hsp27
  • Gene ID: 15507
  • Accession #: P14602
IKBa (P-Ser32)
  • Gene Symbol: Nfkbia Ikba
  • Gene ID: 18035
  • Accession #: Q9Z1E3
JAK1 (P-Tyr1022)
  • Gene Symbol: JAK1 JAK1A JAK1B
  • Gene ID: 3716
  • Accession #: P23458
JAK2 (P-Tyr1007/1008)
  • Gene Symbol: JAK2
  • Gene ID: 3717
  • Accession #: O60674
JNK (P-Thr183)
  • Gene Symbol: Mapk8 Jnk1 Prkm8
  • Gene ID: 26419
  • Accession #: Q91Y86
MEK (P-Ser217/221)
  • Gene Symbol: MAP2K1 MEK1 PRKMK1
  • Gene ID: 5604
  • Accession #: Q02750
MKK3 (P-Ser189)
  • Gene Symbol: MAP2K3 MEK3 MKK3 PRKMK3 SKK2
  • Gene ID: 5606
  • Accession #: P46734
MKK6 (P-Ser207) (MEK6)
  • Gene Symbol: MAP2K6 MEK6 MKK6 PRKMK6 SKK3
  • Gene ID: 5608
  • Accession #: P52564
MSK1 (P-Ser376)
  • Gene Symbol: RPS6KA5 MSK1
  • Gene ID: 9252;
  • Accession #: O75582
MSK2 (P-Ser360)
  • Gene Symbol: RPS6KA4 MSK2
  • Gene ID: 8986
  • Accession #: O75676
mTOR (P-Ser2448)
  • Gene Symbol: MTOR FRAP FRAP1 FRAP2 RAFT1 RAPT1
  • Gene ID: 2475
  • Accession #: P42345
NF-kB (P-Ser536)
  • Gene Symbol: RELA NFKB3
  • Gene ID: 5970
  • Accession #: Q04206
p27 (P-Thr198)
  • Gene Symbol: Cdkn1b
  • Gene ID: 12576
  • Accession #: P46414
p38 (P-Thr180/Tyr182)
  • Gene Symbol: Mapk14 Crk1 Csbp1 Csbp2
  • Gene ID: 26416
  • Accession #: P47811
P53 (P-Ser15)
  • Gene Symbol: Tp53 P53 Trp53
  • Gene ID: 22059
  • Accession #: P02340
P70S6K (P-Thr421/Ser424)
  • Gene Symbol: RPS6KB1 STK14A
  • Gene ID: 6198
  • Accession #: P23443
PDK1 (P-Ser241)
  • Gene Symbol: PDPK1 PDK1
  • Gene ID: 5170
  • Accession #: O15530
PRAS40 (P-Thr246)
  • Gene Symbol: AKT1S1 PRAS40
  • Gene ID: 84335
  • Accession #: Q96B36
PTEN (P-Ser380)
  • Gene Symbol: PTEN MMAC1 TEP1
  • Gene ID: 5728
  • Accession #: P60484
Raf-1 (P-Ser301)
  • Gene Symbol: RAF1 RAF
  • Gene ID: 5894
  • Accession #: P04049
RPS6 (P-Ser235/236)
  • Gene Symbol: RPS6 OK/SW-cl.2
  • Gene ID: 6194
  • Accession #: P62753
RSK1 (P-Ser380)
  • Gene Symbol: RPS6KA1 MAPKAPK1A RSK1
  • Gene ID: 6195
  • Accession #: Q15418
RSK2 (P-Ser386)
  • Gene Symbol: RPS6KA3 ISPK1 MAPKAPK1B RSK2
  • Gene ID: 6197
  • Accession #: P51812
SHP-1 (P-Ser591)
  • Gene Symbol: PTPN6 HCP PTP1C
  • Gene ID: 5777
  • Accession #: P29350
SHP2 (P-Tyr542)
  • Gene Symbol: PTPN11 PTP2C SHPTP2
  • Gene ID: 5781
  • Accession #: Q06124
SMAD1 (P-Ser463/465)
  • Gene Symbol: SMAD1 BSP1 MADH1 MADR1
  • Gene ID: 4086;
  • Accession #: Q15797
SMAD2 (P-Ser245/250/255)
  • Gene Symbol: SMAD2 MADH2 MADR2
  • Gene ID: 4087;
  • Accession #: Q15796
SMAD4 (P-Thr277)
  • Gene Symbol: SMAD4 DPC4 MADH4
  • Gene ID: 4089;
  • Accession #: Q13485
SMAD5 (P-Ser463/465)
  • Gene Symbol: SMAD5 MADH5
  • Gene ID: 4090;
  • Accession #: Q99717
Src (P-Tyr419)
  • Gene Symbol: SRC SRC1
  • Gene ID: 6714
  • Accession #: P12931
STAT1 (P-Ser727)
  • Gene Symbol: STAT1
  • Gene ID: 6772
  • Accession #: P42224
STAT2 (P-Tyr689)
  • Gene Symbol: STAT2
  • Gene ID: 6773
  • Accession #: P52630
STAT3 (P-Tyr705)
  • Gene Symbol: STAT3 APRF
  • Gene ID: 6774
  • Accession #: P40763
STAT5 (P-Tyr694)
  • Gene Symbol: STAT5A STAT5
  • Gene ID: 6776
  • Accession #: P42229
STAT6 (P-Tyr641)
  • Gene Symbol: STAT6
  • Gene ID: 6778
  • Accession #: P42226
TAK1 (P-Ser412)
  • Gene Symbol: MAP3K7 TAK1
  • Gene ID: 6885;
  • Accession #: O43318
TBK1 (P-Ser172)
  • Gene Symbol: TBK1 NAK
  • Gene ID: 29110;
  • Accession #: Q9UHD2
TYK2 (P-Tyr1054)
  • Gene Symbol: TYK2
  • Gene ID: 7297
  • Accession #: P29597
ZAP70 (P-Tyr292)
  • Gene Symbol: ZAP70 SRK
  • Gene ID: 7535;
  • Accession #: P43403
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Human Phosphorylation Pathway Profiling Array C55 Membranes
  • Blocking Buffer
  • Detection Antibody Cocktail
  • 500X HRP-Anti-Rabbit IgG Concentrate
  • 20X Wash Buffer I Concentrate
  • 20X Wash Buffer II Concentrate
  • 2X Cell Lysis Buffer Concentrate
  • Detection Buffer C
  • Detection Buffer D
  • 8-Well Incubation Tray w/ Lid
  • Protease Inhibitor Cocktail
  • 100x Phosphatase Inhibitor Cocktail I
  • Phosphatase Inhibitor Cocktail II
  • Plastic Sheets
  • Array Map Template

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Detection Antibody Cocktail
4.Incubate with HRP-Conjugated anti-IgG
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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