Enzymes Recombinant Proteins

Human PHOSPHO2 Recombinant Protein (RPPB2108)

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RPPB2108

Description

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Product Name:Human PHOSPHO2 Recombinant Protein
Product Code:RPPB2108
Size:10µg
Species:Human
Target:PHOSPHO2
Synonyms:Pyridoxal phosphate phosphatase PHOSPHO2, PHOSPHO2.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:The PHOSPHO2 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 10% glycerol and 0.1M NaCl.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMKILLV FDFDNTIIDD NSDTWIVQCA PNKKLPIELR DSYRKGFWTE FMGRVFKYLG DKGVREHEMK RAVTSLPFTP GMVELFNFIR KNKDKFDCII ISDSNSVFID WVLEAASFHD IFDKVFTNPA AFNSNGHLTV ENYHTHSCNR CPKNLCKKVV LIEFVDKQLQ QGVNYTQIVY IGDGGNDVCP VTFLKNDDVA MPRKGYTLQK TLSRMSQNLE PMEYSVVVWS SGVDIISHLQ FLIKD

Pyridoxal phosphate phosphatase PHOSPHO2, orphan 2 (PHOSPHO2) is a member of the haloacid dehalogenase (HAD) superfamily. Phosphatase has an elevated activity toward phosphoethanolamine (PEA) and phosphocholine (PCho). PHOSPHO 1, a phosphoethanolamine/phosphocholine phosphatase, is upregulated in mineralizing cells and is believed to be implicated in the production of inorganic phosphate for bone mineralization. PHOSPHO2 is a recognized phosphatase sharing a 42% sequence identity with PHOSPHO1. PHOSPHO1 and PHOSPHO2 are especially similar, however surprisingly recombinant PHOSPHO2 hydrolyses phosphoethanolamine and phosphocholine comparatively inadequately.

PHOSPHO2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 265 amino acids (1-241) and having a molecular mass of 30.3kDa.PHOSPHO2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:PHOSPHO2: Phosphatase that has high activity toward pyridoxal 5'- phosphate (PLP). Also active at much lower level toward pyrophosphate, phosphoethanolamine (PEA), phosphocholine (PCho), phospho-l-tyrosine, fructose-6-phosphate, p-nitrophenyl phosphate, and h-glycerophosphate. Belongs to the HAD-like hydrolase superfamily. PHOSPHO family.
UniProt Protein Details:

Protein type:EC 3.1.3.74; Phosphatase

Chromosomal Location of Human Ortholog: 2q31.1

Molecular Function:phosphoric monoester hydrolase activity; protein binding

UniProt Code:Q8TCD6
NCBI GenInfo Identifier:74730590
NCBI Gene ID:493911
NCBI Accession:Q8TCD6.1
UniProt Secondary Accession:Q8TCD6,B2RC30, D3DPC7,
UniProt Related Accession:Q8TCD6
Molecular Weight:28kDa
NCBI Full Name:Pyridoxal phosphate phosphatase PHOSPHO2
NCBI Synonym Full Names:phosphatase, orphan 2
NCBI Official Symbol:PHOSPHO2��
NCBI Protein Information:pyridoxal phosphate phosphatase PHOSPHO2
UniProt Protein Name:Pyridoxal phosphate phosphatase PHOSPHO2
Protein Family:Probable phosphatase
UniProt Gene Name:PHOSPHO2��
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q8TCD6
Research Area:
Enzymes
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