Enzymes Recombinant Proteins

Human PHOSPHO1 Recombinant Protein (RPPB2107)

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RPPB2107

Description

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Product Name:Human PHOSPHO1 Recombinant Protein
Product Code:RPPB2107
Size:10µg
Species:Human
Target:PHOSPHO1
Synonyms:Phosphoethanolamine/phosphocholine phosphatase, Phosphatase, Orphan 1, EC 3.1.3.75, Phospho1.
Source:Escherichia Coli
Physical Appearance:Filtered lyophilized (freeze-dried) powder.
Formulation:Filtered (0.4�m) and lyophilized from 0.5mg/ml in 30mM acetate buffer pH-4.
Solubility:It is recommended to add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10�g/ml. In higher concentrations the solubility of this protein is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Stability:Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time.
Purity:Greater than 95% as determined by SDS-PAGE.
Amino Acid Sequence:MRGSHHHHHH GMASMSGCFP VSGLRCLSRD GRMAAQGAPR FLLTFDFDET IVDENSDDSI VRAAPGQRLP ESLRATYREG FYNEYMQRVF KYLGEQGVRP RDLSAIYEAI PLSPGMSDLL QFVAKQGACF EVILISDANT FGVESSLRAA GHHSLFRRIL SNPSGPDARG LLALRPFHTH SCARCPANMC KHKVLSDYLR ERAHDGVHFE RLFYVGDGAN DFCPMGLLAG GDVAFPRRGY PMHRLIQEAQ KAEPSSFRAS VVPWETAADV RLHLQQVLKSC

PHOSPHO1 is involved in mineralization process & plays a role in bone and cartilage matrix mineralization.PHOSPHO1 is expressed at sites of mineralization in bone and cartilage. Highly expressed in osteoblast cell line SaOS-2 which produces a mineralized matrix.Orphan-1 is collagen type -2 is specific for cartilaginous tissues. Orphan1 is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.Phosphoethanolamine (2-O3POCH2CH2NH3) is a key intermediate in the formation of cephalins, it is formed in liver and brain by phosphorylation of ethanolamine.PHOSPHO2 and PHOSPHO1 suggest subtle differences in the charge distributions around the putative substrate entry site and in the location of potential H-bond donors.PHOSPHO1 exhibits high specific phosphoethanolamine and phosphocholine phosphatase activities PHOSPHO1 is a phosphatase enzyme for which expression is upregulated in mineralizing cells. PHOSPHO1 has been implicated in the generation of Pi for matrix mineralization, a process central to skeletal development. PHOSPHO1 is a member of the haloacid dehalogenase (HAD) superfamily of Mg2+-dependent hydrolases. PHOSPHO1 exhibits high specific activities toward phosphoethanolamine (PEA) and phosphocholine (PCho).

Human Phospho1 Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 295 amino acids and having a molecular mass of 31.3 kDa. The Human Phospho1 is fused to a 14 aa His tag at N-Terminus. Human Phosphocholine Phosphatase is purified by proprietary chromatographic techniques.

UniProt Protein Function:PHOSPHO1: Phosphatase that has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho). Involved in the generation of inorganic phosphate for bone mineralization. Belongs to the HAD-like hydrolase superfamily. PHOSPHO family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: EC 3.1.3.75; Lipid Metabolism - glycerophospholipid; PhosphataseChromosomal Location of Human Ortholog: 17q21.32Cellular Component: cytosolMolecular Function: phosphoric monoester hydrolase activity; pyrophosphatase activityBiological Process: phosphatidylcholine biosynthetic process; phosphatidylethanolamine biosynthetic process
UniProt Protein Details:
NCBI Summary:
UniProt Code:Q8TCT1
NCBI GenInfo Identifier:219689097
NCBI Gene ID:162466
NCBI Accession:NP_001137276.1
UniProt Secondary Accession:Q8TCT1,Q17RU6, E9PAM0
UniProt Related Accession:Q8TCT1
Molecular Weight:33.7 kDa
NCBI Full Name:phosphoethanolamine/phosphocholine phosphatase isoform 1
NCBI Synonym Full Names:phosphatase, orphan 1
NCBI Official Symbol:PHOSPHO1��
NCBI Official Synonym Symbols:
NCBI Protein Information:phosphoethanolamine/phosphocholine phosphatase
UniProt Protein Name:Phosphoethanolamine/phosphocholine phosphatase
UniProt Synonym Protein Names:
Protein Family:Probable phosphatase
UniProt Gene Name:PHOSPHO1��
UniProt Entry Name:PHOP1_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q8TCT1
Research Area:
Enzymes
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