Human Phospho-SMAD5 (SER463/465) and Total SMAD5 PharmaGenie ELISA Kit (SBRS1975)

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SKU:
SBRS1975
Product Type:
ELISA Kit
Reactivity:
Human
Applications:
ELISA
ELISA Type:
Sandwich

Description

Human Phospho-SMAD5 (SER463/465) and Total SMAD5 PharmaGenie ELISA Kit (SBRS1975)

Assay Genie Phospho-SMAD5 (Ser463/465) and Total SMAD5 ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated SMAD5 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis. This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-SMAD5 and total SMAD5. An anti-pan SMAD5 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and SMAD5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-SMAD5 (Ser463/465) antibody is used to detect phosphorylated SMAD5 or mouse anti-SMAD5 antibody is used to detect pan SMAD5. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-conjugated anti-mouse IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SMAD5 (Ser463/465) or pan SMAD5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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