Human Phospho-AKT (S473) Quantitative PharmaGenie ELISA Kit (SBRS1744)

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SKU:
SBRS1744
Product Type:
ELISA Kit
Reactivity:
Human
Applications:
ELISA
ELISA Type:
Sandwich

Description

Human Phospho-AKT (S473) Quantitative PharmaGenie ELISA Kit (SBRS1744)

Assay Genie Phospho-AKT(Ser473) Quantitative ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in Human cell lysates. By determining phosphorylated AKT protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis. This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human phospho-AKT. An anti-pan AKT antibody has been coated onto a 96-well plate. Standard and Samples are pipetted into the wells and AKT present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-phospho-AKT(Ser473) antibody is used to detect phosphorylated AKT. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of AKT(Ser473) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm.

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