Human PHF5A Recombinant Protein (RPPB4241)
- SKU:
- RPPB4241
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q7RTV0
Description
| Product Name: | Human PHF5A Recombinant Protein |
| Product Code: | RPPB4241 |
| Size: | 10µg |
| Species: | Human |
| Target: | PHF5A |
| Synonyms: | PHD finger protein 5A, bK223H9.2, INI, Rds3, SAP14b, SF3b14b, PHD finger-like domain-containing protein 5A, PHD finger-like domain protein 5A, Splicing factor 3B-associated 14 kDa protein, PHF5A. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The PHF5A solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 7.5), 0.15M NaCl, 10% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMAKHHPD LIFCRKQAGV AIGRLCEKCD GKCVICDSYV RPCTLVRICD ECNYGSYQGR CVICGGPGVS DAYYCKECTI QEKDRDGCPK IVNLGSSKTD LFYERKKYGF KKR |
PHD Finger Protein 5A, also known as PHF5A, is a part of the PHD-finger superfamily and acts as a chromatin-associated protein. PHF5A is a subunit of the splicing factor 3b protein complex which along with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron�s branch site in a sequence-independent way and anchors the U2 snRNP to the pre-mRNA. PHF5A holds a PHD-finger-like domain which is flanked by highly basic N- and C-termini.
PHF5A Human Recombinant produced in E. coli is a single polypeptide chain containing 133 amino acids (1-110) and having a molecular mass of 14.8kDa. PHF5A is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PHF5A: Acts as a transcriptional regulator by binding to the GJA1/Cx43 promoter and enhancing its up-regulation by ESR1/ER- alpha. Also involved in pre-mRNA splicing. Belongs to the PHF5 family. |
| UniProt Protein Details: | Protein type:RNA-binding; RNA splicing Chromosomal Location of Human Ortholog: 22q13.2 Cellular Component: nucleoplasm; nuclear matrix; nuclear speck; snRNP U2; U12-dependent spliceosome Molecular Function:DNA binding; transcription factor activity Biological Process: nuclear mRNA splicing, via spliceosome; transcription, DNA-dependent; RNA splicing; positive regulation of transcription, DNA-dependent; gene expression |
| NCBI Summary: | This gene encodes a subunit of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. The protein encoded by this gene contains a PHD-finger-like domain that is flanked by highly basic N- and C-termini. This protein belongs to the PHD-finger superfamily and may act as a chromatin-associated protein. This gene has several pseudogenes on different chromosomes. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q7RTV0 |
| NCBI GenInfo Identifier: | 46577625 |
| NCBI Gene ID: | 84844 |
| NCBI Accession: | Q7RTV0.1 |
| UniProt Secondary Accession: | Q7RTV0,Q9UH06, |
| UniProt Related Accession: | Q7RTV0 |
| Molecular Weight: | Calculated MW: 12kDaObserved MW: 14kDa |
| NCBI Full Name: | PHD finger-like domain-containing protein 5A |
| NCBI Synonym Full Names: | PHD finger protein 5A |
| NCBI Official Symbol: | PHF5A�� |
| NCBI Official Synonym Symbols: | INI; Rds3; SF3B7; SAP14b; SF3b14b; bK223H9.2�� |
| NCBI Protein Information: | PHD finger-like domain-containing protein 5A; PHD-finger 5a; splicing factor 3b, subunit 7; PHD finger-like domain protein 5A; splicing factor 3B associated 14 kDa protein; splicing factor 3B-associated 14 kDa protein |
| UniProt Protein Name: | PHD finger-like domain-containing protein 5A |
| UniProt Synonym Protein Names: | Splicing factor 3B-associated 14 kDa protein; SF3b14b |
| UniProt Gene Name: | PHF5A�� |
| UniProt Entry Name: | PHF5A_HUMAN |
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