Enzymes Recombinant Proteins

Human PGK2 Recombinant Protein (RPPB2605)

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SKU:
RPPB2605
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P07205
Research Area:
Enzymes

Description

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Product Name:Human PGK2 Recombinant Protein
Product Code:RPPB2605
Size:5µg
Species:Human
Target:PGK2
Synonyms:Phosphoglycerate kinase 2, dJ417L20.2, PGKB, PGKPSS, Phosphoglycerate kinase, testis specific
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The PGK2 solution (0.5mg/ml) contains 20% glycerol, 20mM Tris-HCl buffer (pH 8.0), 1mM DTT and 0.1M NaCl.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MSLSKKLTLD KLDVRGKRVI MRVDFNVPMK KNQITNNQRI KASIPSIKYC LDNGAKAVVL MSHLGRPDGV PMPDKYSLAP VAVELKSLLG KDVLFLKDCV GAEVEKACAN PAPGSVILLE NLRFHVEEEG KGQDPSGKKI KAEPDKIEAF RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPHKASGFLM KKELDYFAKA LENPVRPFLA ILGGAKVADK IQLIKNMLDK VNEMIIGGGM AYTFLKVLNN MEIGASLFDE EGAKIVKDIM AKAQKNGVRI TFPVDFVTGD KFDENAQVGK ATVASGISPG WMGLDCGPES NKNHAQVVAQ ARLIVWNGPL GVFEWDAFAK GTKALMDEIV KATSKGCITV IGGGDTATCC AKWNTEDKVS HVSTGGGASL ELLEGKILPG�VEALSNM
Biological Activity:Specific activity > 500unit/mg. One unit will convert 1 umole of 1,3-Bisphosphoglycerate to 3-PGA per minute at pH 8.0 at 37?C.

PGK2, also known as Phosphoglycerate kinase 2, is a testis-specific form of phosphoglycerate kinase.Originally, it was assumed that PGK2was a pseudogene. However, nowadays it is known that this protein is a functional phosphoglycerate kinase. During the Embden-Meyerhof-Parnas pathway of glycolysis, in thelater stages of spermatogenesis, the protein catalyses the reversible conversion of 1, 3- bisphosphoglycerate to 3-phosphoglycerate.

PGK2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 437 amino acids (1-417a.a.) and having a molecular mass of 46.9kDa.PGK2 is fused to a 20 amino acid His tag at N-Terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:PGK2: This gene is intronless, arose via retrotransposition of the phosphoglycerate kinase 1 gene, and is expressed specifically in the testis. Initially assumed to be a pseudogene, the encoded protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis.[provided by RefSeq, May 2010]
UniProt Protein Details:

Protein type:Carbohydrate Metabolism - glycolysis and gluconeogenesis; Kinase, other; EC 2.7.2.3

Chromosomal Location of Human Ortholog: 6p12.3

Cellular Component: nucleus

Molecular Function:ATP binding; phosphoglycerate kinase activity

Biological Process: phosphorylation

NCBI Summary:This gene is intronless, arose via retrotransposition of the phosphoglycerate kinase 1 gene, and is expressed specifically in the testis. Initially assumed to be a pseudogene, the encoded protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis.[provided by RefSeq, May 2010]
UniProt Code:P07205
NCBI GenInfo Identifier:21264485
NCBI Gene ID:5232
NCBI Accession:P07205.3
UniProt Secondary Accession:P07205,Q9H107, B2R6Y8,
UniProt Related Accession:P07205
Molecular Weight:44,796 Da
NCBI Full Name:Phosphoglycerate kinase 2
NCBI Synonym Full Names:phosphoglycerate kinase 2
NCBI Official Symbol:PGK2��
NCBI Official Synonym Symbols:PGKB; PGKPS; HEL-S-272; dJ417L20.2��
NCBI Protein Information:phosphoglycerate kinase 2
UniProt Protein Name:Phosphoglycerate kinase 2
UniProt Synonym Protein Names:Phosphoglycerate kinase, testis specific
Protein Family:Phosphoglycerate kinase
UniProt Gene Name:PGK2��
UniProt Entry Name:PGK2_HUMAN
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