Enzymes Recombinant Proteins
Human PGK1 Recombinant Protein (RPPB2601)
- SKU:
- RPPB2601
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P00558
- Research Area:
- Enzymes
Description
| Product Name: | Human PGK1 Recombinant Protein |
| Product Code: | RPPB2601 |
| Size: | 25µg |
| Species: | Human |
| Target: | PGK1 |
| Synonyms: | Phosphoglycerate kinase 1, Primer recognition protein 2, Cell migration-inducing gene 10 protein, PRP 2, PGKA, MIG10, MGC8947, MGC117307, MGC142128, PGK1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The PGK1 solution containing 20mM Tris (pH 8.0), 10% Glycerol and 1mM DTT. |
| Stability: | PGK1 although stable at 4°C for 1 week, should be stored below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MSLSNKLTLD KLDVKGKRVV MRVDFNVPMK NNQITNNQRI KAAVPSIKFC LDNGAKSVVL MSHLGRPDGV PMPDKYSLEP VAVELKSLLG KDVLFLKDCV GPEVEKACAN PAAGSVILLE NLRFHVEEEG KGKDASGNKV KAEPAKIEAF RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPQKAGGFLM KKELNYFAKA LESPERPFLA ILGGAKVADK IQLINNMLDK VNEMIIGGGM AFTFLKVLNN MEIGTSLFDE EGAKIVKDLM SKAEKNGVKI TLPVDFVTAD KFDENAKTGQ ATVASGIPAG WMGLDCGPES SKKYAEAVTR AKQIVWNGPV GVFEWEAFAR GTKALMDEVV KATSRGCITI IGGGDTATCC AKWNTEDKVS HVSTGGGASL ELLEGKVLPG VDALSNI |
PGK1 is an X-linked enzyme that has a major role in the glycolytic pathway. PGK1 is a glycolytic enzyme which catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, generating an ATP molecule. PGK1 may also act as a cofactor for polymerase alpha. Defects in the PGK1 gene are usually associated with chronic hemolytic anemia, though it can be accompanied by either mental retardation or muscular disease (rhabdomyolysis). Overexpression of PGK1 and its signalling targets are possibly an expression-pathway in diffuse primary gastric carcinomas promoting peritoneal dissemination. It was shown that PGK1 is differentially expressed in the dorsolateral prefrontal cortex from patients with schizophrenia.
PGK1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 437 amino acids (1-417 a.a.) and having a molecular mass of 46.8kDa. PGK1 is fused to 20 a.a. His-Tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PGK1: an apparent multifunctional protein. A glycolytic enzyme and a polymerase alpha cofactor protein (primer recognition protein). |
| UniProt Protein Details: | Protein type:Carbohydrate Metabolism - glycolysis and gluconeogenesis; Kinase, other; EC 2.7.2.3 Chromosomal Location of Human Ortholog: Xq13.3 Cellular Component: membrane; cytosol Molecular Function:protein binding; phosphoglycerate kinase activity; ATP binding Biological Process: glycolysis; epithelial cell differentiation; carbohydrate metabolic process; glucose metabolic process; pathogenesis; phosphorylation; gluconeogenesis Disease: Phosphoglycerate Kinase 1 Deficiency |
| NCBI Summary: | The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome. [provided by RefSeq, Jan 2014] |
| UniProt Code: | P00558 |
| NCBI GenInfo Identifier: | 52788229 |
| NCBI Gene ID: | 5230 |
| NCBI Accession: | P00558.3 |
| UniProt Secondary Accession: | P00558,Q5J7W1, Q6IBT6, Q8NI87, A8K4W6, B7Z7A9, |
| UniProt Related Accession: | P00558 |
| Molecular Weight: | 417 |
| NCBI Full Name: | Phosphoglycerate kinase 1 |
| NCBI Synonym Full Names: | phosphoglycerate kinase 1 |
| NCBI Official Symbol: | PGK1�� |
| NCBI Official Synonym Symbols: | PGKA; MIG10; HEL-S-68p�� |
| NCBI Protein Information: | phosphoglycerate kinase 1; PRP 2; primer recognition protein 2; cell migration-inducing gene 10 protein; epididymis secretory sperm binding protein Li 68p |
| UniProt Protein Name: | Phosphoglycerate kinase 1 |
| UniProt Synonym Protein Names: | Cell migration-inducing gene 10 protein; Primer recognition protein 2; PRP 2 |
| Protein Family: | Phosphoglycerate kinase |
| UniProt Gene Name: | PGK1�� |
| UniProt Entry Name: | PGK1_HUMAN |
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