Growth Factors & Cytokines Recombinant Proteins

Human Periostin Recombinant Protein (RPPB0851)

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SKU:
RPPB0851
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q15063
Research Area:
Growth Factors & Cytokines

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human Periostin Recombinant Protein
Product Code:RPPB0851
Size:10µg
Species:Human
Target:Periostin
Synonyms:OSF-2, Periostin, Osteoblast Specific Factor 2, PN OSF-2, PDLPOSTN, POSTN, MGC119510, MGC119511, PN, RP11-412K4.1.
Source:HEK293 Cells
Physical Appearance:Filtered White lyophilized (freeze-dried) powder.
Formulation:Periostin was filtered (0.4 �m) and lyophilized from 0.5mg/ml solution in phosphate buffered saline and 5% trehalose.
Solubility:It is recommended to add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Periostin is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Stability:Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time.
Purity:Greater than 38.0% as determined by SDS-PAGE.
Amino Acid Sequence:NNHYDKILAH SRIRGRDQGP NVCALQQILG TKKKYFSTCK NWYKKSICGQ KTTVLYECCP GYMRMEGMKG CPAVLPIDHV YGTLGIVGAT TTQRYSDASK LREEIEGKGS FTYFAPSNEA WDNLDSDIRR GLESNVNVEL LNALHSHMIN KRMLTKDLKN GMIIPSMYNN LGLFINHYPN GVVTVNCARI IHGNQIATNG VVHVIDRVLT QIGTSIQDFI EAEDDLSSFR AAAITSDILE ALGRDGHFTL FAPTNEAFEK LPRGVLERIM GDKVASEALM KYHILNTLQC SESIMGGAVF ETLEGNTIEI GCDGDSITVN GIKMVNKKDI VTNNGVIHLI DQVLIPDSAK QVIELAGKQQ TTFTDLVAQL GLASALRPDG EYTLLAPVNN AFSDDTLSMD QRLLKLILQN HILKVKVGLN ELYNGQILET IGGKQLRVFV YRTAVCIENS CMEKGSKQGR NGAIHIFREI IKPAEKSLHE KLKQDKRFST FLSLLEAADL KELLTQPGDW TLFVPTNDAF KGMTSEEKEI LIRDKNALQN IILYHLTPGV FIGKGFEPGV TNILKTTQGS KIFLKEVNDT LLVNELKSKE SDIMTTNGVI HVVDKLLYPA DTPVGNDQLL EILNKLIKYI QIKFVRGSTF KEIPVTVYTT KIITKVVEPK IKVIEGSLQP IIKTEGPTLT KVKIEGEPEF RLIKEGETIT EVIHGEPIIK KYTKIIDGVP VEITEKETRE ERIITGPEIK YTRISTGGGE TEETLKKLLQ EEVTKVTKFI EGGDGHLFED EEIKRLLQGD TPVRKLQANK KVQGSRRRLR EGRSQHHHHH H

Periostin is a disulfide linked 90 kDa, 811 amino acid protein originally isolated as a osteoblast-specific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta-1(TGFbeta1) and bone morphogenetic protein (BMP-2). OSF-2 has a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain and a C-terminal domain. The fourfold repeated domain of OSF-2 shows homology with the insect protein fasciclinPeriostin mRNA is expressed in the developing mouse embryonic and fetal heart, and that it is localized to the endocardial cushions that ultimately divide the primitive heart tube into a four-chambered heart.

Periostin Human Recombinant produced in HEK cells is a single, glycosylated, polypeptide chain (Asn22-Gln836) containing a total of 821 amino acids, having a calculated molecular mass of 91.8kDa and fused to a 6 aa His tag at C-Terminus.

UniProt Protein Function:POSTN: Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Cell adhesion; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 13q13.3

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; trans-Golgi network

Molecular Function:heparin binding; protein binding; cell adhesion molecule binding

Biological Process: tissue development; extracellular matrix organization and biogenesis; regulation of Notch signaling pathway; cell adhesion; skeletal development

NCBI Summary:This gene encodes a secreted extracellular matrix protein that functions in tissue development and regeneration, including wound healing, and ventricular remodeling following myocardial infarction. The encoded protein binds to integrins to support adhesion and migration of epithelial cells. This protein plays a role in cancer stem cell maintenance and metastasis. Mice lacking this gene exhibit cardiac valve disease, and skeletal and dental defects. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]
UniProt Code:Q15063
NCBI GenInfo Identifier:93138709
NCBI Gene ID:10631
NCBI Accession:Q15063.2
UniProt Secondary Accession:Q15063,Q15064, Q29XZ0, Q3KPJ5, Q5VSY5, Q8IZF9, B1ALD8 C0IMJ1, C0IMJ2, C0IMJ4, D2KRH7, F5H628,
UniProt Related Accession:Q15063
Molecular Weight:80,357 Da
NCBI Full Name:Periostin
NCBI Synonym Full Names:periostin, osteoblast specific factor
NCBI Official Symbol:POSTN��
NCBI Official Synonym Symbols:PN; OSF2; OSF-2; PDLPOSTN; periostin��
NCBI Protein Information:periostin; periodontal ligament-specific periostin; osteoblast specific factor 2 (fasciclin I-like)
UniProt Protein Name:Periostin
UniProt Synonym Protein Names:Osteoblast-specific factor 2; OSF-2
Protein Family:Periostin
UniProt Gene Name:POSTN��
UniProt Entry Name:POSTN_HUMAN
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Cell Biology Recombinant Proteins

system_update_altDatasheetHuman Periostin/OSF-2 Recombinant Protein Osteoblast-Specific Factor OSF-2 (POSTN, Periostin, OSF-2) is a secreted, homodimeric protein that belongs to the periostin fam