Human Periodontal Disease Array (20 targets)

Product Sku:	SARB0061
Size:	2, 4, or 8
Species Detected:	Human
Number of Targets Detected:	20
Gene Symbols:	CCL3, CRP, CXCL8, GPNMB, IFNG, IL10, IL12A, IL17A, IL1A, IL1B\|IL2, IL4, IL6, MMP13, MMP9, SPP1, TGFB1, TNF, TNFRSF11A, TNFRSF11B
Compatible Sample Types:	Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:	Sandwich-based
Method of Detection:	Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:	Semi-Quantitative
Solid Support:	Membrane
Suggested Application(s):	Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:	For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Hover over target for more information
IL-1 alpha (IL-1 F1) Gene Symbol: IL1A Gene ID: 3552 Accession #: P01583	IL-1 beta (IL-1 F2) Gene Symbol: IL1B Gene ID: 3553 Accession #: P01584	IL-2 Gene Symbol: IL2 Gene ID: 3558 Accession #: P60568	IL-4 Gene Symbol: IL4 Gene ID: 3565 Accession #: P05112	IL-6 Gene Symbol: IL6 Gene ID: 3569 Accession #: P05231
IL-8 (CXCL8) Gene Symbol: IL8 Gene ID: 3576 Accession #: P10145	IL-10 Gene Symbol: IL10 Gene ID: 3586 Accession #: P22301	IL-12 p70 Gene Symbol: IL12A Gene ID: 3592 Accession #: P29459	IFN-gamma Gene Symbol: IFNG Gene ID: 3458 Accession #: P01579	TGF beta 1 Gene Symbol: TGFB1 Gene ID: 7040 Accession #: P01137
TNF alpha Gene Symbol: TNF Gene ID: 7124 Accession #: P01375	Osteoprotegerin (TNFRSF11B) Gene Symbol: TNFRSF11B Gene ID: 4982 Accession #: O00300	IL-17A Gene Symbol: IL17A Gene ID: 3605 Accession #: Q16552	MIP-1 alpha (CCL3) Gene Symbol: CCL3 Gene ID: 6348 Accession #: P10147	MMP-13 Gene Symbol: MMP13 Gene ID: 4322 Accession #: P45452
MMP-9 Gene Symbol: MMP9 Gene ID: 4318 Accession #: P14780	Osteopontin (SPP1) Gene Symbol: SPP1 Gene ID: 6696 Accession #: P10451	RANK (TNFRSF11A) Gene Symbol: TNFRSF11A Gene ID: 8792 Accession #: Q9Y6Q6	CRP (C-Reactive Protein) Gene Symbol: CRP Gene ID: 1401 Accession #: P02741	Osteoactivin (GPNMB) Gene Symbol: GPNMB Gene ID: 10457 Accession #: Q14956

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

1.	Block membranes
2.	Incubate with Sample
3.	Incubate with Biotinylated Detection Antibody Cocktail
4.	Incubate with HRP-Conjugated Streptavidin
5.	Incubate with Detection Buffers
6.	Image with chemiluminescent imaging system
7.	Perform densitometry and analysis