Enzymes Recombinant Proteins

Human PEPD Recombinant Protein (RPPB2092)

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RPPB2092

Description

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Product Name:Human PEPD Recombinant Protein
Product Code:RPPB2092
Size:20µg
Species:Human
Target:PEPD
Synonyms:Xaa-Pro dipeptidase, X-Pro dipeptidase, Imidodipeptidase, Peptidase D, Proline dipeptidase, Prolidase, PRD, PEPD, Xaa-Pro dipeptidase isoform 1, PROLIDASE.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:PEPD protein solution (0.5mg/ml) containing Phosphate buffered saline (pH7.4), 10% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSHMGSMAAATGP SFWLGNETLK VPLALFALNR QRLCERLRKN PAVQAGSIVV LQGGEETQRY CTDTGVLFRQESFFHWAFGV TEPGCYGVID VDTGKSTLFV PRLPASHATW MGKIHSKEHF KEKYAVDDVQ YVDEIASVLTSQKPSVLLTL RGVNTDSGSVCREASFDGIS KFEVNNTILH PEIVECRVFK TDMELEVLRYTNKISSEAHR EVMKAVKVGM KEYELESLFE HYCYSRGGMR HSSYTCICGS GENSAVLHYG HAGAPNDRTIQNGDMCLFDM GGEYYCFASD ITCSFPANGK FTADQKAVYE AVLRSSRAVM GAMKPGVWWP DMHRLADRIH LEELAHMGILSGSVDAMVQA HLGAVFMPHG LGHFLGIDVH DVGGYPEGVE RIDEPGLRSL RTARHLQPGM VLTVEPGIYFIDHLLDEALA DPARASFLNR EVLQRFRGFG GVRIEEDVVV TDSGIELLTC VPRTVEEIEA CMAGCDKAFTPFSGPK

Peptidase D, also known as PEPD, Is a part of the peptidase family. PEPD is involved in collagen metabolism due to the high level of iminoacids in collagen. PEPD recycles proline, and sets the pace for the production of collagen. PEPD is also parts dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position.

PEPD Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 516 amino acids (1-493a.a.) and having a molecular mass of 56.9kDa.PEPD is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:peptidase D: Splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. Plays an important role in collagen metabolism because the high level of iminoacids in collagen. Defects in PEPD are a cause of prolidase deficiency (PD). Prolidase deficiency is an autosomal recessive disorder associated with iminodipeptiduria. The clinical phenotype includes skin ulcers, mental retardation, recurrent infections, and a characteristic facies. These features, however are incompletely penetrant and highly variable in both age of onset and severity. There is a tight linkage between the polymorphisms of prolidase and the myotonic dystrophy trait. Belongs to the peptidase M24B family. Eukaryotic-type prolidase subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 3.4.13.9; Protease

Chromosomal Location of Human Ortholog: 19q13.11

Molecular Function:dipeptidase activity; manganese ion binding; aminopeptidase activity; metallocarboxypeptidase activity

Biological Process: amino acid metabolic process; collagen catabolic process; proteolysis

Disease: Prolidase Deficiency

NCBI Summary:This gene encodes a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]
UniProt Code:P12955
NCBI GenInfo Identifier:50403718
NCBI Gene ID:5184
NCBI Accession:P12955.3
UniProt Secondary Accession:P12955,Q8TBN9, Q9BT75, A8K3Z1, A8K416, A8K696, A8MX47 B4DDB7, B4DGJ1, E9PCE8,
UniProt Related Accession:P12955
Molecular Weight:493
NCBI Full Name:Xaa-Pro dipeptidase
NCBI Synonym Full Names:peptidase D
NCBI Official Symbol:PEPD��
NCBI Official Synonym Symbols:PROLIDASE��
NCBI Protein Information:xaa-Pro dipeptidase; imidodipeptidase; X-Pro dipeptidase; proline dipeptidase; aminoacyl-L-proline hydrolase
UniProt Protein Name:Xaa-Pro dipeptidase
UniProt Synonym Protein Names:Imidodipeptidase; Peptidase D; Proline dipeptidase; Prolidase
Protein Family:Dipeptidase
UniProt Gene Name:PEPD��
UniProt Entry Name:PEPD_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P12955
Research Area:
Enzymes
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