Enzymes Recombinant Proteins

Human PDXP Recombinant Protein (RPPB2089)

(No reviews yet) Write a Review
SKU:
RPPB2089
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q96GD0
Research Area:
Enzymes

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human PDXP Recombinant Protein
Product Code:RPPB2089
Size:10µg
Species:Human
Target:PDXP
Synonyms:CIN, PLP, PLPP, EC 3.1.3.74.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:1mg/ml solution containing 20mM Tris pH-8, 1mM DTT, 0.1M NaCl & 20% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MARCERLRGA ALRDVLGRAQ GVLFDCDGVL WNGERAVPGA PELLERLARA GKAALFVSNN SRRARPELAL RFARLGFGGL RAEQLFSSAL CAARLLRQRL PGPPDAPGAV FVLGGEGLRA ELRAAGLRLA GDPSAGDGAA PRVRAVLVGY DEHFSFAKLR EACAHLRDPE CLLVATDRDP WHPLSDGSRT PGTGSLAAAV ETASGRQALV VGKPSPYMFE CITENFSIDP ARTLMVGDRL ETDILFGHRC GMTTVLTLTG VSRLEEAQAY LAAGQHDLVP HYYVESIADL TEGLED

PDXP is the active form of vitamin B6 that functions as a coenzyme in preserving biochemical homeostasis. The desired degradation route from PLP to 4-pyridoxic acid involves the dephosphorylation of PLP by PDXP. PDXP shows activity to pyridoxal 5''-phosphate (PLP), pyridoxine 5''-phosphate (PMP) and Pyridoxine 5''-phosphate (PNP), with a highest activity with PLP followed by PNP.

PDXP Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 316 amino acids (1-296 a.a.) and having a molecular mass of 33.8 kDa. The PDXP is fused to 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:PDXP: Protein serine phosphatase that dephosphorylates 'Ser-3' in cofilin and probably also dephosphorylates phospho-serine residues in DSTN. Regulates cofilin-dependent actin cytoskeleton reorganization. Required for normal progress through mitosis and normal cytokinesis. Does not dephosphorylate phospho-threonines in LIMK1. Does not dephosphorylate peptides containing phospho- tyrosine. Pyridoxal phosphate phosphatase. Has some activity towards pyridoxal 5'-phosphate (PLP), pyridoxine 5'-phosphate (PMP) and pyridoxine 5'-phosphate (PNP), with a highest activity with PLP followed by PNP. Homodimer. Ubiquitous. Highly expressed in all the regions of central nerve system except the spinal cord. Also expressed at high level in liver and testis. In fetus, it is weakly expressed in all organs except brain. Inhibited by NaF, Zn(2+), Ca(2+), Mn(2+) and EDTA. Belongs to the HAD-like hydrolase superfamily.
UniProt Protein Details:

Protein type:Cell cycle regulation; Cofactor and Vitamin Metabolism - vitamin B6; EC 3.1.3.3; EC 3.1.3.74; Protein phosphatase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: actin cytoskeleton; cleavage furrow; cytosol; lamellipodium; midbody; plasma membrane

Molecular Function:heat shock protein binding; magnesium ion binding; phosphoprotein phosphatase activity; protein binding; pyridoxal phosphatase activity

Biological Process: actin rod formation; positive regulation of actin filament depolymerization; protein amino acid dephosphorylation; pyridoxal phosphate catabolic process; regulation of cytokinesis; regulation of mitosis

NCBI Summary:Pyridoxal 5-prime-phosphate (PLP) is the active form of vitamin B6 that acts as a coenzyme in maintaining biochemical homeostasis. The preferred degradation route from PLP to 4-pyridoxic acid involves the dephosphorylation of PLP by PDXP (Jang et al., 2003 [PubMed 14522954]).[supplied by OMIM, Mar 2008]
UniProt Code:Q96GD0
NCBI GenInfo Identifier:10092677
NCBI Gene ID:57026
NCBI Accession:NP_064711.1
UniProt Secondary Accession:Q96GD0,Q9UGY2,
UniProt Related Accession:Q96GD0
Molecular Weight:33.7 kDa
NCBI Full Name:pyridoxal phosphate phosphatase
NCBI Synonym Full Names:pyridoxal phosphatase
NCBI Official Symbol:PDXP��
NCBI Official Synonym Symbols:CIN; PLP; dJ37E16.5��
NCBI Protein Information:pyridoxal phosphate phosphatase
UniProt Protein Name:Pyridoxal phosphate phosphatase
UniProt Synonym Protein Names:Chronophin
Protein Family:Pyridoxal phosphate phosphatase
UniProt Gene Name:PDXP��
View AllClose

0 Reviews

View AllClose

Related Products