Growth Factors & Cytokines Recombinant Proteins

Human PDGF CC Recombinant Protein (RPPB0842)

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SKU:
RPPB0842
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9NRA1
Research Area:
Growth Factors & Cytokines

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human PDGF CC Recombinant Protein
Product Code:RPPB0842
Size:20µg
Species:Human
Target:PDGF CC
Synonyms:Platelet Derived Growth Factor C, Spinal Cord-Derived Growth Factor, FALLOTEIN, PDGF-C, VEGF-E, SCDGF, Secretory Growth Factor-Like Protein, Platelet-Derived Growth Factor C, PDGFC.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:Lyophilized from a 0.2�m filtered solution in Acetonitrile and TFA.
Solubility:It is recommended to reconstitute the lyophilized PDGF-CC in sterile 18M-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized PDGF-CC although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF-CC should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity:Greater than 97.0% as determined by SDS-PAGE.
Amino Acid Sequence:MHHHHHHVVD LNLLTEEVRL YSCTPRNFSV SIREELKRTD TIFWPGCLLV KRCGGNCACC LHNCNECQCV PSKVTKKYHE VLQLRPKTGV RGLHKSLTDV ALEHHEECDC VCRGSTGG
Biological Activity:The ED50, as measured in a proliferation assay using mouse NR6R-3T3 cells, is less than 350ng/ml.

Platelet Derived Growth Factor-CC (PDGF-CC) belongs to the PGDF family of growth factors. PDGF-CC binds with high-affinity to PDGF R-a and activates PDGF R-ab heterodimers. During development, PDGF-CC is involved in ductal morphogenesis, cardiovascular smooth muscle cell proliferation; also PDGF-CC is an angiogenic factor. Furthermore, PDGF-CC is expressed in numerous tumors and tumor cell lines, and may be linked with tumorigenesis.

PDGF-CC Human Recombinant (235-345) produced in E.Coli is a disulfide-linked homodimer containing 2x118 amino acids and having a total molecular mass of 26.8kDa.The PDGF-CC is fused to a 7 amino acid His tag [M-HHHHHH] at N-terminal and purified by proprietary chromatographic techniques.

UniProt Protein Function:PDGFC: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen and chemoattractant for cells of mesenchymal origin. Required for normal skeleton formation during embryonic development, especially for normal development of the craniofacial skeleton and for normal development of the palate. Required for normal skin morphogenesis during embryonic development. Plays an important role in wound healing, where it appears to be involved in three stages: inflammation, proliferation and remodeling. Plays an important role in angiogenesis and blood vessel development. Involved in fibrotic processes, in which transformation of interstitial fibroblasts into myofibroblasts plus collagen deposition occurs. The CUB domain has mitogenic activity in coronary artery smooth muscle cells, suggesting a role beyond the maintenance of the latency of the PDGF domain. In the nucleus, PDGFC seems to have additional function. Belongs to the PDGF/VEGF growth factor family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q32

Cellular Component: cell surface; cytoplasm; endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi membrane; plasma membrane

Molecular Function:platelet-derived growth factor receptor binding; protein binding; protein homodimerization activity

Biological Process: blood coagulation; central nervous system development; platelet-derived growth factor receptor signaling pathway; positive regulation of cell migration; positive regulation of DNA replication; positive regulation of fibroblast proliferation; positive regulation of MAP kinase activity; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein amino acid autophosphorylation

NCBI Summary:The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines. This gene product appears to form only homodimers. It differs from the platelet-derived growth factor alpha and beta polypeptides in having an unusual N-terminal domain, the CUB domain. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2010]
UniProt Code:Q9NRA1
NCBI GenInfo Identifier:205830662
NCBI Gene ID:56034
NCBI Accession:Q9NRA1.2
UniProt Secondary Accession:Q9NRA1,Q4W5M9, Q9UL22, B4DU34, B9EGR8,
UniProt Related Accession:Q9NRA1
Molecular Weight:20,609 Da
NCBI Full Name:Platelet-derived growth factor C
NCBI Synonym Full Names:platelet derived growth factor C
NCBI Official Symbol:PDGFC��
NCBI Official Synonym Symbols:SCDGF; FALLOTEIN��
NCBI Protein Information:platelet-derived growth factor C
UniProt Protein Name:Platelet-derived growth factor C
UniProt Synonym Protein Names:Fallotein; Spinal cord-derived growth factor; SCDGF
Protein Family:Platelet-derived growth factor
UniProt Gene Name:PDGFC��
UniProt Entry Name:PDGFC_HUMAN
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