Growth Factors & Cytokines Recombinant Proteins

Human PDGF AB Recombinant Protein (RPPB0836)

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RPPB0836

Description

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Product Name:Human PDGF AB Recombinant Protein
Product Code:RPPB0836
Size:10µg
Species:Human
Target:PDGF AB
Synonyms:Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, PDGF-AB.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:Lyophilized from 10mM AcOH (Acetic Acid).
Solubility:It is recommended to reconstitute the lyophilized Platelet-derived Growth Factor-AB in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized Platelet-derived Growth Factor AB although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF-AB should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE analysis.
Amino Acid Sequence:Alpha chain: MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPTBeta chain: SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIGIVRK KPIFKKATVT LGDHLACKCE TVAAARPVT
Biological Activity:The ED50 as determined by the dose-dependent proliferation of mouse 3T3 indicator cells, is 1.4-2.1 ng/ml. This corresponds to a specific activity of 7.1x 105 units/mg.

The term �PDGF� refers to a family of disulphide bond-linked dimeric isoforms that act as autocrine and paracrine growth factors and are produced by a variety of cell types other than platelets.They act as potent mitogens for almost all mesenchymally-derived cells. Aberrant expression is involved in certain cancers, fibroproliferative disorders and atherosclerosis. The protein also contributes to wound healing and neural regeneration. There are four members of the PDGF family � PDGF A, PDGF B, PDGF C and PDGF D. Two distinct types of PDGF-A exist � a short form that is soluble and a long form that is retained by the extracellular matrix.

Platelet-derived Growth Factor AB Human Recombinant is a heterodimeric, non-glycosylated, polypeptide chain containing 234 amino acids (and an N-terminal Met) consisting of 14.3kDa alpha-chain and 12.1 beta-chain having a total molecular mass of 26.4kDa.� PDGF-AB is purified by proprietary chromatographic techniques.

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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Research Area:
Growth Factors & Cytokines
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