Human PCNA PharmaGenie ELISA Kit (SBRS0975)
- SKU:
- SBRS0975
Description
system_update_altDatasheet
Human PCNA PharmaGenie ELISA Kit (SBRS0975)
| Product SKU: | SBRS0975 |
| Size: | 96T |
| Application: | The antibody pair provided in this kit recognizes Human PCNA. |
| Uniprot: | P12004 |
| Gene ID: | 5111 |
| Gene Names: | PCNA |
| Synonyms: | Proliferating cell nuclear antigen (PCNA) (Cyclin) |
| Target Species: | Human |
| Compatible Sample Types: | Cell Culture Supernatants, Plasma, Serum |
| Design Principle: | Sandwich-based |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Range: | 2.5 ng/ml - 600 ng/mL |
| Sensitivity: | 2.46 ng/ml |
| Recommended Dilution: | 2 fold |
| Storage/Stability: | May be stored for up to 6 months at 2 to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2 to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles. |
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other materials and equipment required:
The Assay Genie Human PCNA PharmaGenie ELISA Kit (SBRS0975) will require other equipment and materials to carry out the assay. Please see list below for further details.
- Distilled or deionized water
- Precision pipettes to deliver 2 ul to 1 ul volumes
- Adjustable 1-25 ul pipettes for reagent preparation
- 100 ul and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 ul of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 ul of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 ul of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 ul of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 ul of Stop Solution to each well.
- Read at 450 nm immediately.
Additional Information
Product Type: |
ELISA Kit |
Reactivity: |
Human |
Applications: |
ELISA |
ELISA Type: |
Sandwich |
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