Human PAR-1 / Proteinase-activated receptor 1 ELISA Kit
- SKU:
- HUFI00814
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Uniprot:
- P25116
- Sensitivity:
- 0.094ng/ml
- Range:
- 0.156-10ng/ml
- ELISA Type:
- Sandwich
- Synonyms:
- PAR1, Protease Activated Receptor 1, PAR-1, F2R, CF2R, TR, Proteinase-activated receptor 1, Coagulation factor II receptor, Thrombin receptor
- Reactivity:
- Human
- Research Area:
- Cardiovascular
Description
Human PAR-1 / Proteinase-activated receptor 1 ELISA Kit
Proteinase Activated Receptor 1 (PAR1) is a protein that is activated by the enzyme proteinase. PAR1 is found in the endoplasmic reticulum and has been shown to be involved in atherosclerosis and tumor metastasis. PAR1 also plays a key role in cell adhesion, cell migration, and inflammatory responses. It also functions in the immune system by helping to regulate inflammation. The Assay Genie Human PAR1 ELISA kit is a highly sensitive assay for the quantitative measurement of PAR1 in serum, blood, plasma, cell culture supernatant, and tissue samples.
| Product Name: | Human PAR-1 / Proteinase-activated receptor 1 ELISA Kit |
| Product Code: | HUFI00814 |
| Size: | 96 Assays |
| Alias: | PAR1, Protease Activated Receptor 1, PAR-1, F2R, CF2R, TR, Proteinase-activated receptor 1, Coagulation factor II receptor, Thrombin receptor |
| Detection method: | Sandwich ELISA, Double Antibody |
| Application: | This immunoassay kit allows for the in vitro quantitative determination of Human F2R concentrations in serum plasma and other biological fluids. |
| Sensitivity: | 0.094ng/ml |
| Range: | 0.156-10ng/ml |
| Storage: | 4°C for 6 months |
| Note: | For Research Use Only |
| Recovery: | Matrices listed below were spiked with certain level of Human F2R and the recovery rates were calculated by comparing the measured value to the expected amount of Human F2R in samples. | ||||||||||||||||
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| Linearity: | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human F2R and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. | ||||||||||||||||
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| CV(%): | Intra-Assay: CV<8% Inter-Assay: CV<10% |
| Component | Quantity | Storage |
| ELISA Microplate (Dismountable) | 8×12 strips | 4°C for 6 months |
| Lyophilized Standard | 2 | 4°C/-20°C |
| Sample/Standard Dilution Buffer | 20ml | 4°C |
| Biotin-labeled Antibody(Concentrated) | 120ul | 4°C (Protect from light) |
| Antibody Dilution Buffer | 10ml | 4°C |
| HRP-Streptavidin Conjugate(SABC) | 120ul | 4°C (Protect from light) |
| SABC Dilution Buffer | 10ml | 4°C |
| TMB Substrate | 10ml | 4°C (Protect from light) |
| Stop Solution | 10ml | 4°C |
| Wash Buffer(25X) | 30ml | 4°C |
| Plate Sealer | 5 | - |
Other materials and equipment required:
- Microplate reader with 450 nm wavelength filter
- Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
- Incubator
- Deionized or distilled water
- Absorbent paper
- Buffer resevoir
| Uniprot | P25116 |
| UniProt Protein Function: | PAR1: a G-protein coupled high-affinity receptor for activated thrombin or trypsin. Coupled to G proteins that stimulate phosphoinositide hydrolysis. Coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelet activation and in vascular development. |
| UniProt Protein Details: | Protein type:GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR; Cell development/differentiation Chromosomal Location of Human Ortholog: 5q13 Cellular Component: Golgi apparatus; postsynaptic membrane; integral to plasma membrane; early endosome; plasma membrane; extracellular region; caveola; neuromuscular junction Molecular Function:G-protein coupled receptor activity; protein binding; G-protein beta-subunit binding; G-protein alpha-subunit binding; thrombin receptor activity; receptor binding Biological Process: elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of blood coagulation; activation of MAPKK activity; negative regulation of glomerular filtration; positive regulation of transcription, DNA-dependent; positive regulation of collagen biosynthetic process; positive regulation of JAK-STAT cascade; positive regulation of caspase activity; response to lipopolysaccharide; negative regulation of cell proliferation; elevation of cytosolic calcium ion concentration; connective tissue replacement during inflammatory response; positive regulation of MAPKKK cascade; platelet dense granule organization and biogenesis; regulation of interleukin-1 beta production; protein kinase C activation; positive regulation of cell proliferation; response to wounding; positive regulation of smooth muscle contraction; negative regulation of neuron apoptosis; establishment of synaptic specificity at neuromuscular junction; inflammatory response; STAT protein nuclear translocation; caspase activation; platelet activation; anatomical structure morphogenesis; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of Rho protein signal transduction; positive regulation of phosphoinositide 3-kinase cascade; tyrosine phosphorylation of STAT protein; G-protein coupled receptor protein signaling pathway; homeostasis of number of cells within a tissue; release of sequestered calcium ion into cytosol; regulation of blood coagulation; positive regulation of vasoconstriction; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); regulation of sensory perception of pain; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of calcium ion transport; blood coagulation; positive regulation of cell migration |
| NCBI Summary: | Coagulation factor II receptor is a 7-transmembrane receptor involved in the regulation of thrombotic response. Proteolytic cleavage leads to the activation of the receptor. F2R is a G-protein coupled receptor family member. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P25116 |
| NCBI GenInfo Identifier: | 20178318 |
| NCBI Gene ID: | 2149 |
| NCBI Accession: | P25116.2 |
| UniProt Secondary Accession: | P25116,Q53XV0, Q96RF7, Q9BUN4, |
| UniProt Related Accession: | P25116 |
| Molecular Weight: | Calculated: 47kDaObserved: 47kDa |
| NCBI Full Name: | Proteinase-activated receptor 1 |
| NCBI Synonym Full Names: | coagulation factor II (thrombin) receptor |
| NCBI Official Symbol: | F2R |
| NCBI Official Synonym Symbols: | TR; HTR; CF2R; PAR1; PAR-1 |
| NCBI Protein Information: | proteinase-activated receptor 1; protease-activated receptor 1 |
| UniProt Protein Name: | Proteinase-activated receptor 1 |
| UniProt Synonym Protein Names: | Coagulation factor II receptor; Thrombin receptor |
| Protein Family: | Proteinase-activated receptor |
| UniProt Gene Name: | F2R |
| UniProt Entry Name: | PAR1_HUMAN |
*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Before adding to wells, equilibrate the SABC working solution and TMB substrate for at least 30 min at 37°C. When diluting samples and reagents, they must be mixed completely and evenly. It is recommended to plot a standard curve for each test.
| Step | Protocol |
| 1. | Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample in duplicate. Wash plate 2 times before adding standard, sample and control (zero) wells! |
| 2. | Aliquot 0.1ml standard solutions into the standard wells. |
| 3. | Add 0.1 ml of Sample / Standard dilution buffer into the control (zero) well. |
| 4. | Add 0.1 ml of properly diluted sample ( Human serum, plasma, tissue homogenates and other biological fluids.) into test sample wells. |
| 5. | Seal the plate with a cover and incubate at 37 °C for 90 min. |
| 6. | Remove the cover and discard the plate content, clap the plate on the absorbent filter papers or other absorbent material. Do NOT let the wells completely dry at any time. Wash plate X2. |
| 7. | Add 0.1 ml of Biotin- detection antibody working solution into the above wells (standard, test sample & zero wells). Add the solution at the bottom of each well without touching the side wall. |
| 8. | Seal the plate with a cover and incubate at 37°C for 60 min. |
| 9. | Remove the cover, and wash plate 3 times with Wash buffer. Let wash buffer rest in wells for 1 min between each wash. |
| 10. | Add 0.1 ml of SABC working solution into each well, cover the plate and incubate at 37°C for 30 min. |
| 11. | Remove the cover and wash plate 5 times with Wash buffer, and each time let the wash buffer stay in the wells for 1-2 min. |
| 12. | Add 90 µl of TMB substrate into each well, cover the plate and incubate at 37°C in dark within 10-20 min. (Note: This incubation time is for reference use only, the optimal time should be determined by end user.) And the shades of blue can be seen in the first 3-4 wells (with most concentrated standard solutions), the other wells show no obvious color. |
| 13. | Add 50 µl of Stop solution into each well and mix thoroughly. The color changes into yellow immediately. |
| 14. | Read the O.D. absorbance at 450 nm in a microplate reader immediately after adding the stop solution. |
When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.
| Sample Type | Protocol |
| Serum | If using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. |
| Plasma | Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit. |
| Urine & Cerebrospinal Fluid | Collect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid. |
| Cell culture supernatant | Collect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately. |
| Cell lysates | Solubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C. |
| Tissue homogenates | The preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C. |
| Tissue lysates | Rinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C. |
| Breast Milk | Collect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles. |
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