Enzymes Recombinant Proteins

Human PAICS Recombinant Protein (RPPB2068)

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RPPB2068

Description

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Product Name:Human PAICS Recombinant Protein
Product Code:RPPB2068
Size:20µg
Species:Human
Target:PAICS
Synonyms:PAICS, Phosphoribosylaminoimidazole Carboxylase Phosphoribosylaminoimidazole, Succinocarboxamide Synthetase, PAIS, AIRC, ADE2, ADE2H1, AIR Carboxylase, Multifunctional Protein ADE2, Multifunctional Protein ADE2H1, SAICAR Synthetase.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The PAICS solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1M NaCl and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMATAEVL NIGKKLYEGK TKEVYELLDS PGKVLLQSKD QITAGNAARK NHLEGKAAIS NKITSCIFQL LQEAGIKTAF TRKCGETAFI APQCEMIPIE WVCRRIATGS FLKRNPGVKE GYKFYPPKVE LFFKDDANND PQWSEEQLIA AKFCFAGLLI GQTEVDIMSH ATQAIFEILE KSWLPQNCTL VDMKIEFGVD VTTKEIVLAD VIDNDSWRLW PSGDRSQQKD KQSYRDLKEV TPEGLQMVKK NFEWVAERVE LLLKSESQCR VVVLMGSTSD LGHCEKIKKA CGNFGIPCEL RVTSAHKGPD ETLRIKAEYE GDGIPTVFVA VAGRSNGLGP VMSGNTAYPV ISCPPLTPDW GVQDVWSSLR LPSGLGCSTV LSPEGSAQFA AQIFGLSNHL VWSKLRASIL NTWISLKQAD KKIRECNL

Phosphoribosylaminoimidazole Carboxylase (PAICS) is an enzyme involved in nucleotide biosynthesis and particularly in purine biosynthesis. PAICS is a bifunctional enzyme containing phosphoribosylaminoimidazole carboxylase activity at its N-terminal region and phosphoribosylaminoimidazole succinocarboxamide synthetase at its C-terminal region. PAICS catalyzes the conversion of 5'-phosphoribosyl-5-aminoimidazole(AIR) into 5'-phosphoribosyl-4-carboxy-5-aminoimidazole (CAIR) as described in the reaction. PAICS catalyzes steps six and seven of purine biosynthesis.

PAICS Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 448 amino acids (1-425) and having a molecular mass of 49.5kDa.PAICS is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:PAICS: 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Nucleotide Metabolism - purine; EC 6.3.2.6; Ligase; EC 4.1.1.21; Lyase

Chromosomal Location of Human Ortholog: 4q12

Cellular Component: membrane; cytosol

Molecular Function:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity; identical protein binding; protein binding; phosphoribosylaminoimidazole carboxylase activity; ATP binding

Biological Process: purine ribonucleoside monophosphate biosynthetic process; purine base biosynthetic process; nucleobase, nucleoside and nucleotide metabolic process; purine base metabolic process; 'de novo' IMP biosynthetic process

NCBI Summary:This gene encodes a bifunctional enzyme containing phosphoribosylaminoimidazole carboxylase activity in its N-terminal region and phosphoribosylaminoimidazole succinocarboxamide synthetase in its C-terminal region. It catalyzes steps 6 and 7 of purine biosynthesis. The gene is closely linked and divergently transcribed with a locus that encodes an enzyme in the same pathway, and transcription of the two genes is coordinately regulated. The human genome contains several pseudogenes of this gene. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P22234
NCBI GenInfo Identifier:131628
NCBI Gene ID:10606
NCBI Accession:P22234.3
UniProt Secondary Accession:P22234,Q68CQ5, E9PDH9,
UniProt Related Accession:P22234
Molecular Weight:425
NCBI Full Name:Multifunctional protein ADE2
NCBI Synonym Full Names:phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase
NCBI Official Symbol:PAICS��
NCBI Official Synonym Symbols:ADE2; AIRC; PAIS; ADE2H1��
NCBI Protein Information:multifunctional protein ADE2; AIR carboxylase; SAICAR synthetase; multifunctional protein ADE2H1
UniProt Protein Name:Multifunctional protein ADE2
UniProt Synonym Protein Names:SAICAR synthetase
Protein Family:Multifunctional protein
UniProt Gene Name:PAICS��
UniProt Entry Name:PUR6_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P22234
Research Area:
Enzymes
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