Growth Factors & Cytokines Recombinant Proteins

Human OPG Fc Recombinant Protein (RPPB0816)

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RPPB0816

Description

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Product Name:Human OPG Fc Recombinant Protein
Product Code:RPPB0816
Size:50µg
Species:Human
Target:OPG Fc
Synonyms:TNFRSF11B, OPG, OCIF, Osteoclastogenesis inhibitory factor, TR1, MGC29565.
Source:Pichia Pastoris
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:OPG was lyophilized from a 0.2�m filtered concentrated solution in 20mM PB, pH 6.0, 150mM NaCl and 0.02 % Tween-80.
Solubility:It is recommended to reconstitute the lyophilized Osteoprotegerin in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized Osteoprotegerin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution OCIF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity:Greater than 90.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Amino Acid Sequence:OPG 22-201�ETFPPKYLHY DEETSHQLLC DKCPPGTYLK QHCTAKWKTV CAPCPDHYYT DSWHTSDECL YCSPVCKELQ YVKQECNRTH NRVCECKEGR YLEIEFCLKH RSCPPGFGVV QAGTPERNTV CKRCPDGFFS NETSSKAPCR KHTNCSVFGL LLTQKGNATH DNICSGNSES TQKCGIDVTL�Fc232EPKSSDKTHT CPPCPAPEFE GAPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPTPIEKTISKAKGQPRE PQVYTLPPSR DELTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GK
Biological Activity:The ED50 as determined by neutralizing the stimulation of U937 cells is less than 10 ng/ml, corresponding to a specific activity of > 100,000IU/mg in the presence of 10ng/ml soluble human RANKL (sRANKL).�

Osteoprotegerin acts as decoy receptor for rankl and thereby neutralizes its function in osteoclastogenesis. OPG inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local rankl/opg ratio. Osteoprotegerin may also play a role in preventing arterial calcification. May act as decoy receptor for trail and protect against apoptosis. Trail binding blocks the inhibition of osteoclastogenesis.

Recombinant OPG produced in yeast contains 2x412 amino acid residues, including 180 residues from mature OPG (a.a 22-201) and 232 residues from the Fc protein of human IgG1, and has a calculated molecular mass of 109.6kDa.�

UniProt Protein Function:TNFRSF11B: Acts as decoy receptor for RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local RANKL/OPG ratio. May also play a role in preventing arterial calcification. May act as decoy receptor for TRAIL and protect against apoptosis. TRAIL binding blocks the inhibition of osteoclastogenesis. Defects in TNFRSF11B are the cause of juvenile Paget disease (JPD); also known as hyperostosis corticalis deformans juvenilis or hereditary hyperphosphatasia or chronic congenital idiopathic hyperphosphatasia. JPD is a rare autosomal recessive osteopathy that presents in infancy or early childhood. The disorder is characterized by rapidly remodeling woven bone, osteopenia, debilitating fractures, and deformities due to a markedly accelerated rate of bone remodeling throughout the skeleton. Approximately 40 cases of JPD have been reported worldwide. Unless it is treated with drugs that block osteoclast- mediated skeletal resorption, the disease can be fatal.
UniProt Protein Details:

Protein type:Secreted; Secreted, signal peptide; Inhibitor

Chromosomal Location of Human Ortholog: 8q24

Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region

Molecular Function:cytokine activity; receptor activity

Biological Process: response to drug; extracellular matrix organization and biogenesis; response to magnesium ion; negative regulation of odontogenesis of dentine-containing teeth; response to estrogen stimulus; apoptosis; response to arsenic; negative regulation of bone resorption; signal transduction; skeletal development; response to nutrient

Disease: Paget Disease, Juvenile

NCBI Summary:The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined. [provided by RefSeq, Jul 2008]
UniProt Code:O00300
NCBI GenInfo Identifier:322510101
NCBI Gene ID:4982
NCBI Accession:O00300.3
UniProt Secondary Accession:O00300,O60236, Q53FX6, Q9UHP4, B2R9A8,
UniProt Related Accession:O00300
Molecular Weight:46,026 Da
NCBI Full Name:Tumor necrosis factor receptor superfamily member 11B
NCBI Synonym Full Names:tumor necrosis factor receptor superfamily, member 11b
NCBI Official Symbol:TNFRSF11B��
NCBI Official Synonym Symbols:OPG; TR1; OCIF��
NCBI Protein Information:tumor necrosis factor receptor superfamily member 11B; osteoprotegerin; osteoclastogenesis inhibitory factor
UniProt Protein Name:Tumor necrosis factor receptor superfamily member 11B
UniProt Synonym Protein Names:Osteoclastogenesis inhibitory factor; Osteoprotegerin
UniProt Gene Name:TNFRSF11B��
UniProt Entry Name:TR11B_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
O00300
Research Area:
Growth Factors & Cytokines
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