Enzymes Recombinant Proteins

Human OLA1 Recombinant Protein (RPPB2058)

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RPPB2058

Description

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Product Name:Human OLA1 Recombinant Protein
Product Code:RPPB2058
Size:20µg
Species:Human
Target:OLA1
Synonyms:Obg-like ATPase 1, DNA damage-regulated overexpressed in cancer 45, DOC45, GTP-binding protein 9, OLA1, GTPBP9, PRO2455, PTD004, GBP45, GTBP9.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:The OLA1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol and 0.1M NaCl.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles.
Purity:Greater than 95% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMPPKKG GDGIKPPPII GRFGTSLKIG IVGLPNVGKS TFFNVLTNSQ ASAENFPFCT IDPNESRVPV PDERFDFLCQ YHKPASKIPA FLNVVDIAGL VKGAHNGQGL GNAFLSHISA CDGIFHLTRA FEDDDITHVE GSVDPIRDIE IIHEELQLKD EEMIGPIIDK LEKVAVRGGD KKLKPEYDIM CKVKSWVIDQ KKPVRFYHDW NDKEIEVLNK HLFLTSKPMV YLVNLSEKDY IRKKNKWLIK IKEWVDKYDP GALVIPFSGA LELKLQELSA EERQKYLEAN MTQSALPKII KAGFAALQLE YFFTAGPDEV RAWTIRKGTK APQAAGKIHT DFEKGFIMAE VMKYEDFKEE GSENAVKAAG KYRQQGRNYI VEDGDIIFFK FNTPQQPKKK

Obg-like ATPase 1 (OLA1) acts as a negative regulator of the cellular antioxidant response independent of transcriptional processes. OLA1 curbs the antioxidant response via nontranscriptional mechanisms. OLA1 hydrolyzes ATP, and can also hydrolyze GTP with lower efficiency. OLA1 is clearly down-regulated by DNA damage-inducing agents. OLA1 is expressed in all tissues; however it is more abundant in the testis, liver, lung, and brain. OLA1 is overexpressed in a number of malignancies, including cancers of the colon, rectum, ovary, lung, stomach, and uterus.

OLA1 Human Recombinant produced in E. coli is a single polypeptide chain containing 420 amino acids (1-396) and having a molecular mass of 47.3kDa.OLA1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:OLA1: Hydrolyzes ATP, and can also hydrolyze GTP with lower efficiency. Has lower affinity for GTP. Belongs to the GTP1/OBG family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 3.6.3.-; Nucleolus; Hydrolase

Chromosomal Location of Human Ortholog: 2q31.1

Cellular Component: centrosome; cytoplasm; membrane

Molecular Function:ATP binding; ATPase activity; protein binding

Biological Process: ATP metabolic process

NCBI Summary:This gene encodes a member of the GTPase protein family. The encoded protein interacts with breast cancer-associated gene 1 (BRCA1) and BRCA1-associated RING domain protein (BARD1), and is involved in centrosome regulation. Overexpression of this gene has been observed in multiple types of cancer and may be associated with poor survival. Pseudogenes of this gene have been defined on chromosomes 17 and 22. [provided by RefSeq, Jun 2016]
UniProt Code:Q9NTK5
NCBI GenInfo Identifier:25453240
NCBI Gene ID:29789
NCBI Accession:Q9NTK5.2
UniProt Secondary Accession:Q9NTK5,Q5BJD7, Q8NCI8, Q96CU1, Q96SV2, Q9P1D3, Q9UNY9 Q9Y6G4, D7EHM2,
UniProt Related Accession:Q9NTK5
Molecular Weight:31,440 Da
NCBI Full Name:Obg-like ATPase 1
NCBI Synonym Full Names:Obg-like ATPase 1
NCBI Official Symbol:OLA1��
NCBI Official Synonym Symbols:DOC45; GBP45; GTBP9; GTPBP9; PTD004��
NCBI Protein Information:obg-like ATPase 1
UniProt Protein Name:Obg-like ATPase 1
UniProt Synonym Protein Names:DNA damage-regulated overexpressed in cancer 45; DOC45; GTP-binding protein 9
Protein Family:Obg-like ATPase
UniProt Gene Name:OLA1��
UniProt Entry Name:OLA1_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9NTK5
Research Area:
Enzymes
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