Human OBFC1 Recombinant Protein (RPPB4150)
- SKU:
- RPPB4150
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q9H668
Description
| Product Name: | Human OBFC1 Recombinant Protein |
| Product Code: | RPPB4150 |
| Size: | 10µg |
| Species: | Human |
| Target: | OBFC1 |
| Synonyms: | AAF-44, RPA-32, STN1, CST complex subunit STN1, Suppressor of cdc thirteen homolog, Oligonucleotide/oligosaccharide-binding fold-containing protein 1, OBFC1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The OBFC1 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMQPGSSR CEEETPSLLW GLDPVFLAFA KLYIRDILDM KESRQVPGVF LYNGHPIKQV DVLGTVIGVR ERDAFYSYGV DDSTGVINCI CWKKLNTESV SAAPSAAREL SLTSQLKKLQ ETIEQKTKIE IGDTIRVRGS IRTYREEREI HATAYYKVDD PVWNIQIARM LELPTIYRKV YDQPFHSSAL EKEEALSNPG ALDLPSLTSL LSEKAKEFLM ENRVQSFYQQ ELEMVESLLS LANQPVIHSA CSDQVNFKKD TTSKAIHSIF KNAIQLLQEK GLVFQKDDGF DNLYYVTRED KDLHRKIHRI IQQDCQKPNH MEKGCHFLHI LACARLSIRP GLSEAVLQQV LELLEDQSDI VSTMEHYYTA F |
Oligonucleotide Binding Fold Containing 1 (OBFC1) is a part of the CST complex, a complex which binds to single-stranded DNA and is essential to defend telomeres from DNA degradation. The CST complex binds single-stranded DNA with high affinity in a sequence-independent way, whereas isolated subunits bind DNA with low affinity by themselves. The role of the CST complex is less specific in DNA metabolism at non-telomeric sites.
OBFC1 Human Recombinant produced in E. coli is. a single polypeptide chain containing 391 amino acids (1-368) and having a molecular mass of 44.5kDa. OBFC1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | OBFC1: Component of the CST complex, a complex that binds to single-stranded DNA and is required to protect telomeres from DNA degradation. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity by themselves. In addition to telomere protection, the CST complex has probably a more general role in DNA metabolism at non-telomeric sites. Belongs to the STN1 family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 10q24.33 Cellular Component: intermediate filament cytoskeleton; intracellular membrane-bound organelle; nuclear chromosome, telomeric region; nucleolus; nucleoplasm; nucleus Molecular Function:protein binding; single-stranded DNA binding; single-stranded telomeric DNA binding; telomeric DNA binding Biological Process: negative regulation of telomerase activity; positive regulation of DNA replication; positive regulation of telomerase activity; positive regulation of telomere maintenance via telomerase; telomere maintenance |
| NCBI Summary: | OBFC1 and C17ORF68 (MIM 613129) are subunits of an alpha accessory factor (AAF) that stimulates the activity of DNA polymerase-alpha-primase (see MIM 176636), the enzyme that initiates DNA replication (Casteel et al., 2009 [PubMed 19119139]). OBFC1 also appears to function in a telomere-associated complex with C17ORF68 and TEN1 (C17ORF106; MIM 613130) (Miyake et al., 2009 [PubMed 19854130]).[supplied by OMIM, Nov 2009] |
| UniProt Code: | Q9H668 |
| NCBI GenInfo Identifier: | 62900737 |
| NCBI Gene ID: | 79991 |
| NCBI Accession: | Q9H668.2 |
| UniProt Secondary Accession: | Q9H668,Q5TCZ0, D3DR99, |
| UniProt Related Accession: | Q9H668 |
| Molecular Weight: | 42,119 Da |
| NCBI Full Name: | CST complex subunit STN1 |
| NCBI Synonym Full Names: | oligonucleotide/oligosaccharide binding fold containing 1 |
| NCBI Official Symbol: | OBFC1�� |
| NCBI Official Synonym Symbols: | STN1; AAF44; AAF-44; RPA-32; bA541N10.2�� |
| NCBI Protein Information: | CST complex subunit STN1 |
| UniProt Protein Name: | CST complex subunit STN1 |
| UniProt Synonym Protein Names: | Oligonucleotide/oligosaccharide-binding fold-containing protein 1; Suppressor of cdc thirteen homolog |
| Protein Family: | CST complex |
| UniProt Gene Name: | OBFC1�� |
| UniProt Entry Name: | STN1_HUMAN |
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