Enzymes Recombinant Proteins

Human NUDT14 Recombinant Protein (RPPB5822)

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RPPB5822

Description

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Product Name:Human NUDT14 Recombinant Protein
Product Code:RPPB5822
Size:20µg
Species:Human
Target:NUDT14
Synonyms:UGPP, UGPPase, Uridine diphosphate glucose pyrophosphatase, UDPG pyrophosphatase, Nucleoside diphosphate-linked moiety X motif 14, Nudix motif 14, NUDT14.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The NUDT14 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 10% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMERIEGA SVGRCAASPY LRPLTLHYRQ NGAQKSWDFM KTHDSVTVLL FNSSRRSLVL VKQFRPAVYA GEVERRFPGS LAAVDQDGPR ELQPALPGSA GVTVELCAGL VDQPGLSLEE VACKEAWEEC GYHLAPSDLR RVATYWSGVG LTGSRQTMFY TEVTDAQRSG PGGGLVEEGE LIEVVHLPLE GAQAFADDPD IPKTLGVIFG VSWFLSQVAP NLDLQ

Uridine diphosphate glucose pyrophosphatase (NUDT14), is a part of the nudix hydrolase family. NUDT14 is a cytoplasmic protein which contains one nudix hydrolase domain and acts as the sugar donor in numerous glycosylation reactions, including those involved in the production of glycogen. NUDT14 hydrolyzes ADP-ribose into ribose 5-phosphate and AMP, and UDP-glucose to glucose 1-phosphate and UMP. NUDT14 is a homodimer which binds magnesium as a cofactor and is encoded by a gene located on human chromosome 14.

NUDT14 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 245 amino acids (1-222) and having a molecular mass of 26.5kDa. NUDT14 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:NUDT14: Hydrolyzes UDP-glucose to glucose 1-phosphate and UMP and ADP-ribose to ribose 5-phosphate and AMP. The physiological substrate is probably UDP-glucose. Poor activity on other substrates such as ADP-glucose, CDP-glucose, GDP-glucose and GDP- mannose. Belongs to the Nudix hydrolase family.Protein type: Hydrolase; EC 3.6.1.45Chromosomal Location of Human Ortholog: 14q32.33Cellular Component: cytoplasmMolecular Function: protein binding; ADP-ribose diphosphatase activity; metal ion binding; UDP-sugar diphosphatase activityBiological Process: metabolic process
UniProt Protein Details:
NCBI Summary:UDP-glucose (UDPG) acts as the sugar donor in numerous glycosylation reactions, including those involved in the production of glycogen. NUDT14 is a UDPG pyrophosphatase (EC 3.6.1.45) that hydrolyzes UDPG to produce glucose 1-phosphate and UMP (Yagi et al., 2003 [PubMed 12429023]).[supplied by OMIM, Mar 2008]
UniProt Code:O95848
NCBI GenInfo Identifier:37594467
NCBI Gene ID:256281
NCBI Accession:NP_803877.2
UniProt Secondary Accession:O95848,Q86SJ8
UniProt Related Accession:O95848
Molecular Weight:24,118 Da
NCBI Full Name:uridine diphosphate glucose pyrophosphatase
NCBI Synonym Full Names:nudix (nucleoside diphosphate linked moiety X)-type motif 14
NCBI Official Symbol:NUDT14��
NCBI Official Synonym Symbols:UGPP; UGPPase��
NCBI Protein Information:uridine diphosphate glucose pyrophosphatase; UDPG pyrophosphatase; UDP-sugar diphosphatase
UniProt Protein Name:Uridine diphosphate glucose pyrophosphatase
UniProt Synonym Protein Names:Nucleoside diphosphate-linked moiety X motif 14; Nudix motif 14
Protein Family:Nudix hydrolase
UniProt Gene Name:NUDT14��
UniProt Entry Name:NUD14_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
O95848
Research Area:
Enzymes
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