Human Nuclear Factor Related To Kappa B Binding Protein (NFRkB) ELISA Kit
(No reviews yet)
Write a Review
- SKU:
- HUDL02029
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Uniprot:
- Q6P4R8
- Sensitivity:
- 0.127ng/mL
- Range:
- 0.312-20ng/mL
- ELISA Type:
- Sandwich
- Synonyms:
- INO80G,DNA-Binding Protein R Kappa B,INO80 Complex Subunit G
- Reactivity:
- Human
- Sample Type:
- Tissue Homogenates And Other Biological Fluids
Description
system_update_altDatasheet
| Product Name: | Human Nuclear Factor Related To Kappa B Binding Protein (NFRkB) ELISA Kit |
| Product Code: | HUDL02029 |
| Size: | 96 Assays |
| Target: | NFRkB |
| Synonyms: | INO80G, DNA-Binding Protein R Kappa B, INO80 Complex Subunit G |
| Detection Method: | Sandwich |
| Range: | 0.312-20ng/mL |
| Shelf Life: | 12 months |
| Note: | For research use only |
| Recovery: | Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples. | ||||||||||||||||||||
| |||||||||||||||||||||
| Linearity: | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. | ||||||||||||||||||||
| |||||||||||||||||||||
| Intra-assay Precision: | Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively. | ||||||||||||||||||||
| Inter-assay Precision: | Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate. | ||||||||||||||||||||
| Stability: | The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end. |
| Step | Protocol |
| 1. | Prepare all reagents, samples and standards |
| 2. | Add 100µL standard or sample to each well. Incubate 2 hours at 37°C |
| 3. | Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C |
| 4. | Aspirate and wash 3 times |
| 5. | Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C |
| 6. | Aspirate and wash 5 times |
| 7. | Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C |
| 8. | Add 50µL Stop Solution. Read at 450nm immediately. |
Related Products
Human NFRkB (Nuclear Factor Related To Kappa B Binding Protein) CLIA Kit (HUES01259)
Human Epigenetics and Nuclear Signaling ELISA Kits
Human Nuclear Factor Kappa B / NkB ELISA Kit
Human Nuclear Factor Kappa B / NkB ELISANuclear Factor Kappa B / NF-?B is a transcription factor that translocates to the nucleus upon activation and controls the expression of genes involved in...
