Human NSMCE1 Recombinant Protein (RPPB4127)

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RPPB4127

Description

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Product Name:Human NSMCE1 Recombinant Protein
Product Code:RPPB4127
Size:20µg
Species:Human
Target:NSMCE1
Synonyms:Non-SMC Element 1 Homolog (S. Cerevisiae), NSE1, Non-Structural Maintenance of Chromosomes Element 1 Homolog, Non-SMC Element 1 Homolog, EC 6.3.2.-.�
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:The NSMCE1 solution contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 1mM DTT and 20% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks.�Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMQGSTRR MGVMTDVHRR FLQLLMTHGV LEEWDVKRLQ THCYKVHDRN ATVDKLEDFI NNINSVLESL YIEIKRGVTE DDGRPIYALV NLATTSISKM ATDFAENELD LFRKALELII DSETGFASST NILNLVDQLK GKKMRKKEAE QVLQKFVQNK WLIEKEGEFT LHGRAILEME QYIRETYPDA VKICNICHSL LIQGQSCETC GIRMHLPCVA KYFQSNAEPR CPHCNDYWPH EIPKVFDPEK ERESGVLKSN KKSLRSRQH

NSMCE1 is a component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT- associated PML bodies (APBs). NSMCE1 has in vitro ubiquitin ligase activity in presence of NDNL2.

NSMCE1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 289 amino acids (1-266) and having a molecular mass of 33.0kDa.NSMCE1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:NSMCE1: Component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT- associated PML bodies (APBs). Has in vitro ubiquitin ligase activity in presence of NDNL2. Is involved in positive regulation of response to DNA damage stimulus. Belongs to the NSE1 family.
UniProt Protein Details:

Protein type:Ubiquitin conjugating system; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 16p12.1

Cellular Component: intracellular membrane-bound organelle; nucleoplasm; nucleus

Molecular Function:protein binding; ubiquitin-protein ligase activity

Biological Process: double-strand break repair via homologous recombination; postreplication repair; protein sumoylation

UniProt Code:Q8WV22
NCBI GenInfo Identifier:209572785
NCBI Gene ID:197370
NCBI Accession:Q8WV22.5
UniProt Secondary Accession:Q8WV22,Q9P045, Q9P049, D3DWF6,
UniProt Related Accession:Q8WV22
Molecular Weight:30,855 Da
NCBI Full Name:Non-structural maintenance of chromosomes element 1 homolog
NCBI Synonym Full Names:NSE1 homolog, SMC5-SMC6 complex component
NCBI Official Symbol:NSMCE1��
NCBI Official Synonym Symbols:NSE1��
NCBI Protein Information:non-structural maintenance of chromosomes element 1 homolog
UniProt Protein Name:Non-structural maintenance of chromosomes element 1 homolog
UniProt Gene Name:NSMCE1��
UniProt Entry Name:NSE1_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q8WV22
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