Human NSL1 Recombinant Protein (RPPB4126)

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RPPB4126

Description

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Product Name:Human NSL1 Recombinant Protein
Product Code:RPPB4126
Size:20µg
Species:Human
Target:NSL1
Synonyms:NSL1 MIS12 Kinetochore Complex Component, NSL1 MIND Kinetochore Complex Component Homolog (S. Cerevisiae), Kinetochore-Associated ProteinNSL1 Homolog, Chromosome 1 Open Reading Frame 48, C1orf48, MIS14.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:The NSL1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMAGSPEL VVLDPPWDKE LAAGTESQAL VSATPREDFR VRCTSKRAVT EMLQLCGRFV QKLGDALPEE IREPALRDAQ WTFESAVQEN ISINGQAWQE ASDNCFMDSD IKVLEDQFDE IIVDIATKRK QYPRKILECV IKTIKAKQEI LKQYHPVVHP LDLKYDPDPA PHMENLKCRG ETVAKEISEA MKSLPALIEQ GEGFSQVLRM QPVIHLQRIH QEVFSSCHRK PDAKPENFIT QIETTPTETA SRKTSDMVLK RKQTKDCPQR KWYPLRPKKI NLDT

NSL1 holds two coiled-coil domains.NSL1 proteins, localized to kinetochores - chromosome associated structures which attach to microtubules and mediate chromosome movements throughout cell division. NSL1 is a fragment of a conserved protein complex that hold two chromodomain-containing proteins and a component of the outer plate of the kinetochore. NSL1 connect centromeric heterochromatin with the outer kinetochore structure. Several transcript variants are known to encod different isoforms for this gene.

NSL1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 304 amino acids (1-281) and having a molecular mass of 34.6kDa.NSL1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:NSL1: Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 1q41

Cellular Component: cytosol; nucleus; MIS12/MIND type complex

Molecular Function:protein binding

Biological Process: mitosis; cell division; mitotic cell cycle; chromosome segregation

NCBI Summary:This gene encodes a protein with two coiled-coil domains that localizes to kinetochores, which are chromosome-associated structures that attach to microtubules and mediate chromosome movements during cell division. The encoded protein is part of a conserved protein complex that includes two chromodomain-containing proteins and a component of the outer plate of the kinetochore. This protein complex is proposed to bridge centromeric heterochromatin with the outer kinetochore structure. Multiple transcript variants encoding different isoforms have been found for this gene. There is a pseudogene of the 3' UTR region of this gene on chromosome X. [provided by RefSeq, Jul 2014]
UniProt Code:Q96IY1
NCBI GenInfo Identifier:147744577
NCBI Gene ID:25936
NCBI Accession:Q96IY1.3
UniProt Secondary Accession:Q96IY1,Q5SY75, Q9H2M5, Q9NRN8, Q9Y415, E7ETD5,
UniProt Related Accession:Q96IY1
Molecular Weight:281
NCBI Full Name:Kinetochore-associated protein NSL1 homolog
NCBI Synonym Full Names:NSL1, MIS12 kinetochore complex component
NCBI Official Symbol:NSL1��
NCBI Official Synonym Symbols:DC8; MIS14; C1orf48��
NCBI Protein Information:kinetochore-associated protein NSL1 homolog; NSL1, MIND kinetochore complex component, homolog
UniProt Protein Name:Kinetochore-associated protein NSL1 homolog
Protein Family:Kinetochore-associated protein
UniProt Gene Name:NSL1��
UniProt Entry Name:NSL1_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q96IY1
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