Enzymes Recombinant Proteins

Human NNMT Recombinant Protein (RPPB2031)

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SKU:
RPPB2031
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P40261
Research Area:
Enzymes

Description

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Product Name:Human NNMT Recombinant Protein
Product Code:RPPB2031
Size:10µg
Species:Human
Target:NNMT
Synonyms:Nicotinamide N-methyltransferase, EC 2.1.1.1, NNMT.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:NNMT protein solution (1mg/ml) containing 20 mM Tris-HCl buffer (pH 8.0) and 20% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MESGFTSKDT YLSHFNPRDY LEKYYKFGSR HSAESQILKH LLKNLFKIFC LDGVKGDLLI DIGSGPTIYQ LLSACESFKE IVVTDYSDQN LQELEKWLKK EPEAFDWSPV VTYVCDLEGN RVKGPEKEEK LRQAVKQVLK CDVTQSQPLG AVPLPPADCV LSTLCLDAAC PDLPTYCRAL RNLGSLLKPG GFLVIMDALK SSYYMIGEQK FSSLPLGREA VEAAVKEAGY TIEWFEVISQ SYSSTMANNE GLFSLVARKL SRPL
Biological Activity:Specific activity is > 100 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37C.

NNMT is part of the family of transferases, especially those transferring one-carbon group methyltransferases. NNMT is mostly expressed in the liver, and a lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. NNMT catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is significant for biotransformation of many drugs and xenobiotic compounds. NNMT is accountable for the enzymatic activity which uses S-adenosyl methionine as the methyl donor. NNMT expression is related with tumor stage and DFS time in hepatocellular carcinoma cases. NNMT is a good candidate as a tumor marker of various kinds of cancers. NNMT serum levels have significance in the premature detection and in the management of patients with colorectal cancer.

NNMT Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 284 amino acids (1-264 a.a) and having a molecular mass of 37.7kDa.NNMT is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:NNMT: Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds. Belongs to the NNMT/PNMT/TEMT family.
UniProt Protein Details:

Protein type:Methyltransferase; EC 2.1.1.1; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide

Chromosomal Location of Human Ortholog: 11q23.1

Cellular Component: cytosol

Molecular Function:nicotinamide N-methyltransferase activity

Biological Process: response to drug; methylation; organ regeneration; xenobiotic metabolic process; response to organic nitrogen

NCBI Summary:N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor. [provided by RefSeq, Jul 2008]
UniProt Code:P40261
NCBI GenInfo Identifier:730163
NCBI Gene ID:4837
NCBI Accession:P40261.1
UniProt Related Accession:P40261
Molecular Weight:
NCBI Full Name:Nicotinamide N-methyltransferase
NCBI Synonym Full Names:nicotinamide N-methyltransferase
NCBI Official Symbol:NNMT��
NCBI Protein Information:nicotinamide N-methyltransferase
UniProt Protein Name:Nicotinamide N-methyltransferase
UniProt Gene Name:NNMT��
UniProt Entry Name:NNMT_HUMAN
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