Enzymes Recombinant Proteins

Human NMNAT2 Recombinant Protein (RPPB2027)

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SKU:
RPPB2027
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9BZQ4
Research Area:
Enzymes

Description

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Product Name:Human NMNAT2 Recombinant Protein
Product Code:RPPB2027
Size:10µg
Species:Human
Target:NMNAT2
Synonyms:Nicotinamide Nucleotide Adenylyltransferase 2, C1orf15, Nicotinate-NucleotideAdenylyltransferase 2, NaMN Adenylyltransferase 2, NMN Adenylyltransferase 2, PNAT2,KIAA0479, Chromosome 1 Open Reading Frame 15, Nicotinamide MononucleotideAdenylyltransferase 2, Pyridine Nucleotide Adenylyltransferase 2, EC 2.7.7.1, EC2.7.7.18.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:NMNAT2 protein solution (0.25mg/ml) containing 20mMTris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSHMTETTKTHVI LLACGSFNPI TKGHIQMFER ARDYLHKTGR FIVIGGIVSP VHDSYGKQGL VSSRHRLIMCQLAVQNSDWI RVDPWECYQD TWQTTCSVLE HHRDLMKRVT GCILSNVNTP SMTPVIGQPQ NETPQPIYQNSNVATKPTAA KILGKVGESL SRICCVRPPV ERFTFVDENA NLGTVMRYEE IELRILLLCG SDLLESFCIPGLWNEADMEV IVGDFGIVVV PRDAADTDRI MNHSSILRKY KNNIMVVKDD INHPMSVVSS TKSRLALQHGDGHVVDYLSQ PVIDYILKSQ LYINASG
Biological Activity:Specific activity: > 500 pmol/min/ug. One unit will convert 1.0 pmole of beta-NADH per minute to beta-NAD at PH 8.0 at 37�C.

NicotinamideNucleotide Adenylyltransferase 2, also known as NMNAT2 is a member of thenicotinamide mononucleotide adenylyltransferase (NMNAT) enzyme family, membersof which catalyze a vital step in NAD (NADP) biosynthetic pathway. Unlike theother human family member, which is localized to the nucleus, and isubiquitously expressed; NMNAT2 is cytoplasmic, and is predominantlyexpressed in the brain. Two transcript variants encoding different isoformshave been found for NMNAT2. Among the diseases associated with NMNAT2 aretauopathy, and systemic lupus erythematosus.

NMNAT2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 327 amino acids (1-307 a.a) and having a molecular mass of 36.6kDa.NMNAT2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:NMNAT2: Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate but with a lower efficiency. Cannot use triazofurin monophosphate (TrMP) as substrate. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity prefers NAD(+), NADH and NAAD as substrates and degrades nicotinic acid adenine dinucleotide phosphate (NHD) less effectively. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NAADP(+). Belongs to the eukaryotic NMN adenylyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 2.7.7.1; EC 2.7.7.18; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Transferase

Chromosomal Location of Human Ortholog: 1q25

Cellular Component: Golgi apparatus; Golgi membrane

Molecular Function:nicotinamide-nucleotide adenylyltransferase activity; nicotinate-nucleotide adenylyltransferase activity

Biological Process: NAD biosynthetic process; NAD metabolic process

NCBI Summary:This gene product belongs to the nicotinamide mononucleotide adenylyltransferase (NMNAT) enzyme family, members of which catalyze an essential step in NAD (NADP) biosynthetic pathway. Unlike the other human family member, which is localized to the nucleus, and is ubiquitously expressed; this enzyme is cytoplasmic, and is predominantly expressed in the brain. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:Q9BZQ4
NCBI GenInfo Identifier:30580486
NCBI Gene ID:23057
NCBI Accession:Q9BZQ4.1
UniProt Secondary Accession:Q9BZQ4,O75067, Q5T1Q3, Q8WU99, Q96QW1,
UniProt Related Accession:Q9BZQ4
Molecular Weight:34,015 Da
NCBI Full Name:Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 2
NCBI Synonym Full Names:nicotinamide nucleotide adenylyltransferase 2
NCBI Official Symbol:NMNAT2��
NCBI Official Synonym Symbols:PNAT2; C1orf15��
NCBI Protein Information:nicotinamide/nicotinic acid mononucleotide adenylyltransferase 2
UniProt Protein Name:Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 2
UniProt Synonym Protein Names:Nicotinamide mononucleotide adenylyltransferase 2; NMN adenylyltransferase 2; Nicotinate-nucleotide adenylyltransferase 2; NaMN adenylyltransferase 2
UniProt Gene Name:NMNAT2��
UniProt Entry Name:NMNA2_HUMAN
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