Enzymes Recombinant Proteins

Human NMNAT1 Recombinant Protein (RPPB5821)

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RPPB5821

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Product Name:Human NMNAT1 Recombinant Protein
Product Code:RPPB5821
Size:10µg
Species:Human
Target:NMNAT1
Synonyms:NMNAT, NMNAT1, PNAT1, Nicotinamide mononucleotide adenylyltransferase 1, NMN adenylyltransferase 1, Nicotinate-nucleotide adenylyltransferase 1, NaMN adenylyltransferase 1, EC=2.7.7.1, EC=2.7.7.18.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:The NMNAT1 solution (1mg/ml) contains 20mM Tris-HClbuffer (pH8.0), 20% glycerol, 0.1M NaCl, 1mM DTT and 1mM EDTA.
Stability:Store at 4°C if entire vial will be used within 2-4weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrierprotein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMENS EKTEVVLLAC GSFNPITNMH LRLFELAKDY MNGTGRYTVV KGIISPVGDA YKKKGLIPAY HRVIMAELAT KNSKWVEVDT WESLQKEWKE TLKVLRHHQE KLEASDCDHQ QNSPTLERPG RKRKWTETQD SSQKKSLEPK TKAVPKVKLL CGADLLESFA VPNLWKSEDI TQIVANYGLI CVTRAGNDAQ KFIYESDVLW KHRSNIHVVN EWIANDISST KIRRALRRGQSIRYLVPDLV QEYIEKHNLY SSESEDRNAG VILAPLQRNT AEAKT
Biological Activity:Specific activity is > 7,000 pmol/min/ug, and wasobtained by measuring the beta-NAD from nicotinamide mononucleotide and ATP perminute at pH 8.0 at 37C.

NMNAT1 enzyme is vital for NAD biosynthesis, catalyzing the condensation of nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) with the AMP moiety of ATP to form NAD or NaAD. NMNAT1 is widely expressed with high levels in skeletal muscle, heart, liver and kidney. This protein appears to have the ability to protect against axonal degeneration following mechanical or toxic insults.

NMNAT1 Recombinant Human produced in E.Coli is a single,non-glycosylated polypeptide chain containing 315 amino acids (1-279 a.a.) andhaving a molecular mass of 36 kDa.�The NMNAT1 is fused to a 36 amino acidHis-Tag at N-terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:NMNAT1: Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency. Can use triazofurin monophosphate (TrMP) as substrate. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity, prefers NAD(+) and NAAD as substrates and degrades NADH, nicotinic acid adenine dinucleotide phosphate (NHD) and nicotinamide guanine dinucleotide (NGD) less effectively. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NAADP(+). Protects against axonal degeneration following mechanical or toxic insults. Belongs to the eukaryotic NMN adenylyltransferase family.
UniProt Protein Details:

Protein type:EC 2.7.7.18; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Transferase; EC 2.7.7.1

Chromosomal Location of Human Ortholog: 1p36.22

Cellular Component: nucleoplasm; nucleus

Molecular Function:protein binding; nicotinamide-nucleotide adenylyltransferase activity; nicotinate-nucleotide adenylyltransferase activity; ATP binding

Biological Process: NAD biosynthetic process; vitamin metabolic process; NAD metabolic process; water-soluble vitamin metabolic process

Disease: Leber Congenital Amaurosis 9

NCBI Summary:This gene encodes an enzyme which catalyzes a key step in the biosynthesis of nicotinamide adenine dinucleotide (NAD). The encoded enzyme is one of several nicotinamide nucleotide adenylyltransferases, and is specifically localized to the cell nucleus. Activity of this protein leads to the activation of a nuclear deacetylase that functions in the protection of damaged neurons. Mutations in this gene have been associated with Leber congenital amaurosis 9. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are located on chromosomes 1, 3, 4, 14, and 15. [provided by RefSeq, Jul 2014]
UniProt Code:Q9HAN9
NCBI GenInfo Identifier:30580491
NCBI Gene ID:64802
NCBI Accession:Q9HAN9.1
UniProt Secondary Accession:Q9HAN9,Q8TAE9, Q9H247, Q9H6B6, B1AN63,
UniProt Related Accession:Q9HAN9
Molecular Weight:279
NCBI Full Name:Nicotinamide mononucleotide adenylyltransferase 1
NCBI Synonym Full Names:nicotinamide nucleotide adenylyltransferase 1
NCBI Official Symbol:NMNAT1��
NCBI Official Synonym Symbols:LCA9; NMNAT; PNAT1��
NCBI Protein Information:nicotinamide mononucleotide adenylyltransferase 1; NMN adenylyltransferase 1; NaMN adenylyltransferase 1; pyridine nucleotide adenylyltransferase 1; nicotinate-nucleotide adenylyltransferase 1
UniProt Protein Name:Nicotinamide mononucleotide adenylyltransferase 1
UniProt Synonym Protein Names:Nicotinate-nucleotide adenylyltransferase 1 (EC:2.7.7.18); NaMN adenylyltransferase 1
UniProt Gene Name:NMNAT1��
UniProt Entry Name:NMNA1_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9HAN9
Research Area:
Enzymes
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