Human NME4 Recombinant Protein (RPPB4111)

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SKU:
RPPB4111
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
O00746

Description

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Product Name:Human NME4 Recombinant Protein
Product Code:RPPB4111
Size:10µg
Species:Human
Target:NME4
Synonyms:Nucleoside diphosphate kinase mitochondrial, Nucleoside diphosphate kinase, mitochondrial, NDK, NDPKD, nm23-H4, NM23D.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The NME4 solution (0.5mg/ml) contains 40% glycerol, 20mM Tris-HCl buffer (pH 8.0) and 0.2M NaCl.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MPSWTRERTL VAVKPDGVQR RLVGDVIQRF ERRGFTLVGM KMLQAPESVL AEHYQDLRRK PFYPALIRYM SSGPVVAMVW EGYNVVRASR AMIGHTDSAE AAPGTIRGDF SVHISRNVIH ASDSVEGAQR EIQLWFQSSE LVSWADGGQH SSIHPA�
Biological Activity:Specific activity is > 120unit/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25C in a couple system with PK/LDH.

Non-Metastatic Cells 4 or NME4, is a nucleoside diphosphate kinase located in the mitochondria, and is part of the NDK family of proteins. NME4 ais a very common enzyme that enhances transfer of gamma-phosphates, through a phosphohistidine as a intermediate, between dioxynucleoside tri- and diphosphates. NME4 is originated from the nm23 gene. NME4 has a crucial part in the creation of nucleoside triphosphates that are not ATP.

NME4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 176 amino acids (33-187a.a.) and having a molecular mass of 19.6kDa.NME4 is fused to a 21 amino acid His tag at N-Terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:NME4: Major role in the synthesis of nucleoside triphosphates other than ATP. Belongs to the NDK family.
UniProt Protein Details:

Protein type:Kinase, nucleoside diphosphate; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; EC 2.7.4.6; Kinase, other; Mitochondrial; Other group; NDK family

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: mitochondrial inner membrane; mitochondrion

Molecular Function:nucleoside diphosphate kinase activity; protein binding

Biological Process: nucleobase, nucleoside and nucleotide interconversion; nucleoside metabolic process; nucleoside triphosphate biosynthetic process; purine nucleotide metabolic process; pyrimidine nucleotide metabolic process; regulation of apoptosis

NCBI Summary:The nucleoside diphosphate (NDP) kinases (EC 2.7.4.6) are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates. The enzymes are products of the nm23 gene family, which includes NME4 (Milon et al., 1997 [PubMed 9099850]).[supplied by OMIM, May 2008]
UniProt Code:O00746
NCBI GenInfo Identifier:2498075
NCBI Gene ID:4833
NCBI Accession:O00746.1
UniProt Secondary Accession:O00746,Q5U0M9, A2IDD0,
UniProt Related Accession:O00746
Molecular Weight:12,979 Da
NCBI Full Name:Nucleoside diphosphate kinase, mitochondrial
NCBI Synonym Full Names:NME/NM23 nucleoside diphosphate kinase 4
NCBI Official Symbol:NME4��
NCBI Official Synonym Symbols:NDPK-D; NM23H4; nm23-H4��
NCBI Protein Information:nucleoside diphosphate kinase, mitochondrial
UniProt Protein Name:Nucleoside diphosphate kinase, mitochondrial
UniProt Synonym Protein Names:Nucleoside diphosphate kinase D; NDPKD; nm23-H4
UniProt Gene Name:NME4��
UniProt Entry Name:NDKM_HUMAN
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