Human NME3 Recombinant Protein (RPPB4109)

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SKU:
RPPB4109
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q13232

Description

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Product Name:Human NME3 Recombinant Protein
Product Code:RPPB4109
Size:10µg
Species:Human
Target:NME3
Synonyms:Nucleoside diphosphate kinase 3, c371H6.2, DR-nm23, KIAA0516, NDPK-C, NDPKC, NM23-H3, NM23H3.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The NME3 solution (0.5mg/ml) contains 50% glycerol, 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl and 2mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MERTFLAVKP DGVQRRLVGE IVRRFERKGF KLVALKLVQA SEELLREHYA ELRERPFYGR LVKYMASGPV VAMVWQGLDV VRTSRALIGA TNPADAPPGT IRGDFCIEVG KNLIHGSDSV ESARREIALW FRADELLCWE DSAGHWLYE
Biological Activity:Specific activity is > 150unit/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25C in a couple system with PK/LDH.

Non-Metastatic Cells 3 or NME3 is a possible suppressor of metastasis. NME3 can be found at a lower levels in greatly metastatic cells when compared to cells that has less metastatic potential NME3� is crucial for the nucleoside triphosphates synthesis, also, in apoptosis induction & hematopoiesis. NME3 is can be expressed during early stages of myeloid differentiation of greatly purified CD34+ cells.

NME3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 169 amino acids (22-169 a.a.) and having a molecular mass of 19.1kDa.NME3 is fused to a 21 amino acid His tag at N-Terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:NME3: Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Probably has a role in normal hematopoiesis by inhibition of granulocyte differentiation and induction of apoptosis. Belongs to the NDK family.
UniProt Protein Details:

Protein type:Nucleotide Metabolism - pyrimidine; EC 2.7.4.6; Apoptosis; Nucleotide Metabolism - purine; Kinase, nucleoside diphosphate; Motility/polarity/chemotaxis; Kinase, other; Other group; NDK family

Chromosomal Location of Human Ortholog: 16q13.3

Cellular Component: mitochondrion; cytosol

Molecular Function:metal ion binding; nucleoside diphosphate kinase activity; ATP binding

Biological Process: GTP biosynthetic process; regulation of apoptosis; CTP biosynthetic process; pyrimidine nucleotide metabolic process; apoptosis; UTP biosynthetic process; nucleoside triphosphate biosynthetic process; nucleoside diphosphate phosphorylation; purine nucleotide metabolic process

UniProt Code:Q13232
NCBI GenInfo Identifier:21264477
NCBI Gene ID:4832
NCBI Accession:Q13232.2
UniProt Secondary Accession:Q13232,Q9BWH4,
UniProt Related Accession:Q13232
Molecular Weight:19,015 Da
NCBI Full Name:Nucleoside diphosphate kinase 3
NCBI Synonym Full Names:NME/NM23 nucleoside diphosphate kinase 3
NCBI Official Symbol:NME3��
NCBI Official Synonym Symbols:NDPKC; NDPK-C; NM23H3; DR-nm23; NM23-H3; c371H6.2��
NCBI Protein Information:nucleoside diphosphate kinase 3; NDK 3; NDP kinase 3; NDP kinase C; non-metastatic cells 3, protein expressed in; nucleoside diphosphate kinase C
UniProt Protein Name:Nucleoside diphosphate kinase 3
UniProt Synonym Protein Names:DR-nm23; Nucleoside diphosphate kinase C; NDPKC; nm23-H3
Protein Family:Nucleoside diphosphate kinase
UniProt Gene Name:NME3��
UniProt Entry Name:NDK3_HUMAN
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