Human NME1 Recombinant Protein (RPPB4106)
- SKU:
- RPPB4106
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P15531
Description
| Product Name: | Human NME1 Recombinant Protein |
| Product Code: | RPPB4106 |
| Size: | 10µg |
| Species: | Human |
| Target: | NME1 |
| Synonyms: | Non-metastatic cells 1, Nucleoside diphosphate kinase A, NDP kinase A, AWD, GAAD, NB, NBS, NDPK-A, NM23, NM23-H1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The NME1 solution (1mg/ml) contains 10% glycerol, 20mM Tris-HCl buffer (pH 7.5) and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MANCERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVGLKF MQASEDLLKE HYVDLKDRPF FAGLVKYMHS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVESAEKEI GLWFHPEELV DYTSCAQNWI YE |
| Biological Activity: | Specific activity is > 1,200unit/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25C in a couple system with PK/LDH. |
Non-metastatic cells 1 or NME1 is a protein, found at first as a suppressor gene for candidate metastasis. The protein can be found in various types of tumor, potential of metastatic may increase or decrease as the protein�s levels changes. When the protein�s concentration is low, an aggressive carcinoma (colon, breast, gastric and melanoma) appears. High levels of NME1 have been linked to advanced thyroid cancer.
NME1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 152 amino acids (1-152 a.a.) and having a molecular mass of 17.1kDa.
| UniProt Protein Function: | NME1: an enzyme with multiple activities including histidine protein kinase, nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase and 3'-5' exonuclease activities. Plays a major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Involved in cell proliferation, differentiation and development, G protein-coupled receptor endocytosis, cytotoxic T cell and NK cell-mediated cell death, and gene expression. Required for neural development including neural patterning and cell fate determination. During granzyme A (GZMA)-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair. Three alternatively spliced human proteins have been reported. |
| UniProt Protein Details: | Protein type:Kinase, other; EC 2.7.4.6; Tumor suppressor; Nucleotide Metabolism - pyrimidine; Kinase, nucleoside diphosphate; Protein kinase, histidine; Nucleotide Metabolism - purine; Other group; NDK family Chromosomal Location of Human Ortholog: 17q21.3 Cellular Component: cytoplasm; cytosol; membrane; nucleus Molecular Function:ATP binding; deoxyribonuclease activity; GTP binding; identical protein binding; magnesium ion binding; nucleoside diphosphate kinase activity; protein binding; ribosomal small subunit binding Biological Process: negative regulation of cell proliferation; nucleobase, nucleoside and nucleotide interconversion; nucleoside triphosphate biosynthetic process; positive regulation of DNA binding; positive regulation of epithelial cell proliferation; purine nucleotide metabolic process; pyrimidine nucleotide metabolic process; regulation of apoptosis Disease: Neuroblastoma, Susceptibility To |
| NCBI Summary: | This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P15531 |
| NCBI GenInfo Identifier: | 127981 |
| NCBI Gene ID: | 4830 |
| NCBI Accession: | P15531.1 |
| UniProt Secondary Accession: | P15531,Q6FGK3, Q86XQ2, Q9UDJ6, |
| UniProt Related Accession: | P15531 |
| Molecular Weight: | 30,137 Da |
| NCBI Full Name: | Nucleoside diphosphate kinase A |
| NCBI Synonym Full Names: | NME/NM23 nucleoside diphosphate kinase 1 |
| NCBI Official Symbol: | NME1�� |
| NCBI Official Synonym Symbols: | NB; AWD; NBS; GAAD; NDKA; NM23; NDPKA; NDPK-A; NM23-H1�� |
| NCBI Protein Information: | nucleoside diphosphate kinase A |
| UniProt Protein Name: | Nucleoside diphosphate kinase A |
| UniProt Synonym Protein Names: | Granzyme A-activated DNase; GAAD; Metastasis inhibition factor nm23; NM23-H1; Tumor metastatic process-associated protein |
| Protein Family: | Nucleoside diphosphate kinase |
| UniProt Gene Name: | NME1�� |
| UniProt Entry Name: | NDKA_HUMAN |
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