Human NKX3-1 Recombinant Protein (RPPB4103)

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SKU:
RPPB4103
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q99801

Description

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Product Name:Human NKX3-1 Recombinant Protein
Product Code:RPPB4103
Size:20µg
Species:Human
Target:NKX3-1
Synonyms:Homeobox protein Nkx-3.1, Homeobox protein NK-3 homolog A, NKX3-1, NKX3.1, NKX3A, NKX3, BAPX2.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:NKX3-1 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 2M UREA and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMLRVPEP RPGEAKAEGA APPTPSKPLT SFLIQDILRD GAQRQGGRTS SQRQRDPEPE PEPEPEGGRS RAGAQNDQLS TGPRAAPEEA ETLAETEPER HLGSYLLDSE NTSGALPRLP QTPKQPQKRS RAAFSHTQVI ELERKFSHQK YLSAPERAHL AKNLKLTETQ VKIWFQNRRY KTKRKQLSSE LGDLEKHSSL PALKEEAFSR ASLVSVYNSY PYYPYLYCVG SWSPAFW

NK3 Homeobox1 (NKX3-1) is a homeobox-containing transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and may act as a transcriptional repressor. The NKX3-1 transcription factor functions as a negative regulator of epithelial cell growth in prostate tissue. NKX3-1 has an imperative role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. In addition, NKX3-1 functions as a tumor suppressor controlling prostate carcinogenesis, as shown by its ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells. Abnormal expression of NKX3-1 is linked with prostate tumor progression.

NKX3-1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 257 amino acids (1-234 a.a) and having a molecular mass of 28.7kDa.NKX3-1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:NKX3-1: Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Play an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Act as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells. Interacts with serum response factor (SRF). Interacts with SPDEF. Interacts with WDR77. Interacts with TOPORS which polyubiquitinates NKX3-1 and induces its proteasomal degradation. By androgens and, in the LNCAP cell line, by estrogens. Androgenic control may be lost in prostate cancer cells during tumor progression from an androgen-dependent to an androgen- independent phase. Highly expressed in the prostate and, at a lower level, in the testis. Belongs to the NK-3 homeobox family. 5 isoforms of the human protein are produced by alternative splicing.Protein type: Tumor suppressor; Transcription factor; DNA-bindingChromosomal Location of Human Ortholog: 8p21.2Cellular Component: intracellular; nucleusMolecular Function: androgen receptor activity; caspase activator activity; estrogen receptor activity; estrogen receptor binding; histone deacetylase binding; protein binding; protein kinase activator activity; protein self-association; sequence-specific DNA binding; transcription factor activity; transcription factor bindingBiological Process: androgen receptor signaling pathway; branching morphogenesis of a tube; caspase activation; multicellular organismal development; negative regulation of cell proliferation; negative regulation of epithelial cell proliferation; negative regulation of insulin-like growth factor receptor signaling pathway; negative regulation of mitotic cell cycle; negative regulation of transcription, DNA-dependent; positive regulation of caspase activity; positive regulation of cell division; positive regulation of cell proliferation; positive regulation of mitotic cell cycle; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein amino acid phosphorylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein kinase B signaling cascade; response to testosterone stimulus; salivary gland development
UniProt Protein Details:
NCBI Summary:This gene encodes a homeobox-containing transcription factor. This transcription factor functions as a negative regulator of epithelial cell growth in prostate tissue. Aberrant expression of this gene is associated with prostate tumor progression. Alternate splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jan 2012]
UniProt Code:Q99801
NCBI GenInfo Identifier:17377578
NCBI Gene ID:4824
NCBI Accession:Q99801.2
UniProt Secondary Accession:Q99801,O15465, Q9H2P4, Q9H2P5, Q9H2P6, Q9H2P7, Q9HBG0
UniProt Related Accession:Q99801
Molecular Weight:21,626 Da
NCBI Full Name:Homeobox protein Nkx-3.1
NCBI Synonym Full Names:NK3 homeobox 1
NCBI Official Symbol:NKX3-1��
NCBI Official Synonym Symbols:NKX3; BAPX2; NKX3A; NKX3.1��
NCBI Protein Information:homeobox protein Nkx-3.1
UniProt Protein Name:Homeobox protein Nkx-3.1
UniProt Synonym Protein Names:Homeobox protein NK-3 homolog A
Protein Family:Homeobox protein
UniProt Gene Name:NKX3-1��
UniProt Entry Name:NKX31_HUMAN
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