Enzymes Recombinant Proteins

Human NIT2 Recombinant Protein (RPPB2023)

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RPPB2023

Description

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Product Name:Human NIT2 Recombinant Protein
Product Code:RPPB2023
Size:10µg
Species:Human
Target:NIT2
Synonyms:Nitrilase homolog 2, Nitrilase family member 2, omega-amidase NIT2, MGC111199, Nit protein 2, EC 3.5.1.3.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:The NIT2 protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH 8.0) 1mM DTT, 0.1M NaCl, and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks.�Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles.
Purity:Greater than 95% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMTSFRLA LIQLQISSIK SDNVTRACSF IREAATQGAK IVSLPECFNS PYGAKYFPEY AEKIPGESTQ KLSEVAKECS IYLIGGSIPE EDAGKLYNTC AVFGPDGTLL AKYRKIHLFD IDVPGKITFQ ESKTLSPGDS FSTFDTPYCR VGLGICYDMR FAELAQIYAQ RGCQLLVYPG AFNLTTGPAH WELLQRSRAV DNQVYVATAS PARDDKASYV AWGHSTVVNP WGEVLAKAGT EEAIVYSDID LKKLAEIRQQ IPVFRQKRSD LYAVEMKKP

NIT2 is a member of the nitrilase superfamily. NIT2 protein has an omega-amidase activity which removes potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. In addition, Nit2 is widely distributed in nature and is thought to be a tumor suppressor protein.

NIT2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 299 amino acids (1-276a.a.) and having a molecular mass of 33kDa.NIT2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:NIT2: a protein of unknown function. A CN hydrolase domain suggests that this protein may be involved in nitrilase-related metabolism and handling.
UniProt Protein Details:

Protein type:EC 3.5.1.3; Hydrolase; Mitochondrial

Chromosomal Location of Human Ortholog: 3q12.2

Cellular Component: centrosome; mitochondrion; cytoplasm

Molecular Function:omega-amidase activity

Biological Process: oxaloacetate metabolic process; glutamine metabolic process; asparagine metabolic process

UniProt Code:Q9NQR4
NCBI GenInfo Identifier:74725271
NCBI Gene ID:56954
NCBI Accession:Q9NQR4.1
UniProt Secondary Accession:Q9NQR4,Q8WUF0, B2R9A3, D3DN47,
UniProt Related Accession:Q9NQR4
Molecular Weight:276
NCBI Full Name:Omega-amidase NIT2
NCBI Synonym Full Names:nitrilase family, member 2
NCBI Official Symbol:NIT2��
NCBI Official Synonym Symbols:HEL-S-8a��
NCBI Protein Information:omega-amidase NIT2; Nit protein 2; nitrilase homolog 2; epididymis secretory sperm binding protein Li 8a
UniProt Protein Name:Omega-amidase NIT2
UniProt Synonym Protein Names:Nitrilase homolog 2
Protein Family:Nitrilase
UniProt Gene Name:NIT2��
UniProt Entry Name:NIT2_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9NQR4
Research Area:
Enzymes
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