Human Neuromedin-U PharmaGenie ELISA Kit (SBRS0096)
- SKU:
- SBRS0096
- Product Type:
- ELISA Kit
- Reactivity:
- Human
- Applications:
- ELISA
- ELISA Type:
- Competitive
Description
Human Neuromedin-U PharmaGenie ELISA Kit (SBRS0096)
| Product SKU: | SBRS0096 |
| Size: | 96T |
| Application: | This ELISA kit is designed to detect human, mouse, and rat Neuromedin-U. |
| Uniprot: | P48645 |
| Gene ID: | 10874 |
| Synonyms: | Neuromedin-U [Cleaved into: Neuromedin-U-25 (NmU-25)] |
| Target Species: | Human, Mouse, Rat |
| Compatible Sample Types: | Cell Culture Supernatants, Plasma, Serum |
| Design Principle: | Competition-based |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Range: | 0.1-1,000 ng/ml |
| Sensitivity: | 0.2 ng/ml |
| Recommended Dilution: | Human: 16x, Mouse: 16x, Rat: 8x |
| Storage/Stability: | Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2 to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge. |
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- Technical Manual
Other materials and equipment required:
The Assay Genie Human Neuromedin-U PharmaGenie ELISA Kit (SBRS0096) will require other equipment and materials to carry out the assay. Please see list below for further details.
- Distilled or deionized water
- Precision pipettes to deliver 2 ul to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
- Prepare all reagents, samples and standards as instructed.
- Add 100 ul detection antibody to each well.
- Incubate 1.5 h at RT or O/N at 4°C.
- Add 100 ul standard or sample to each well.
- Incubate 2.5 h at RT.
- Add 100 ul prepared streptavidin solution.
- Incubate 45 min at RT.
- Add 100 ul TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 ul Stop Solution to each well.
- Read plate at 450 nm immediately.
 | Standard Curve for Human Neuromedin-U PharmaGenie ELISA Kit (SBES0096) |
Neuromedin-U is a 25 amino acids (23 aas in mouse and rat) neuropeptide found in the central nervous system. It regulates smooth muscle contraction in a tissue- and species-specific manner. Neuromedin-U binds to receptors NMUR1 and NMUR2, and activate intracellular signal transduction via calcium mobilization, phosphoinositide (or PI) signaling, and the inhibition of cAMP. Neuromedin-U plays roles in pain perception, stress response and cancer.
Related Products
Human Neuromedin-B PharmaGenie ELISA Kit (SBRS0095)
OverviewProduct SKU:SBRS0095Size:96TApplication:This ELISA kit is designed to detect human, mouse, and rat Neuromedin-B-32.Uniprot:
Human NMU / Neuromedin-U ELISA Kit
system_update_altDatasheet - तकनीकी मैनुअलsystem_update_altMSDS - तकनीक
Mouse Neuromedin-B PharmaGenie ELISA Kit (SBRS0063)
Mouse Neuromedin-B PharmaGenie ELISA Kit (SBRS0063)OverviewProduct SKU:SBRS0063Size:96TApplication:This ELISA kit is designed to detect human, mouse, and