Human Neurokinin-B PharmaGenie ELISA Kit (SBRS0092)

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SKU:
SBRS0092
Product Type:
ELISA Kit
Reactivity:
Human
Applications:
ELISA
ELISA Type:
Competitive

Description

Human Neurokinin-B PharmaGenie ELISA - Kit Information

Neurokinin B is a peptide hormone belonging to the tachykinin family, which also includes Substance P and Neurokinin A. All the peptides from tachykinin families are derived from two preprotachykinin genes - the PPT-A gene and PPT-B gene. The former encodes Substance P. Neurokinin A & Neuropeptide K, and the latter encodes Neurokinin B. Neurokinin B has shown important roles in regulating immune and neurological functions in humans. It is found in higher concentration in women suffering from pre-eclampsia during pregnancy. It is reported to stimulate the production of immunoglobulins in peripheral B lymphocytes via its NK-3 receptor. Recently Neurokinin B, along with its NK-3 receptor, has shown to play a critical role as a key neuroregulatory switcher on human puberty, governed by the brain through the release of the hormone GnRH, which starts a series of processes that ultimately leads to the production of sex hormones. Neurokinin B has shown potential clinical application as a biomarker in pre-eclampsia during pregnancy and onset of puberty.

Human Neurokinin-B PharmaGenie ELISA - Kit Data

Product SKU:SBRS0092
Size:96T
Application:Cross Reactivity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Ghrelin, Nesfatin, Angiotensin II, NPY and APC.
Uniprot:Q9UHF0
Gene ID:6866
Gene Names:NKNB / TAC3 / UNQ585/PRO1155
Synonyms:Tachykinin-3 (ZNEUROK1) [Cleaved into: Neurokinin-B (NKB) (Neuromedin-K)]
Target Species:Human, Mouse, Rat
Compatible Sample Types:Cell Culture Supernatants, Serum
Design Principle:Competition-based
Method of Detection:Colorimetric
Quantitative/Semi-Quantitative:Quantitative
Range:0.1-1,000 pg/ml
Sensitivity:3.7 pg/ml
Recommended Dilution:Human: 2X, Mouse: 2X, Rat: 2X
Storage/Stability:Standard, Biotinylated Neurokinin B peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2 to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Kit Components:

1.Pre-Coated 96-well Strip Microplate
2.Wash Buffer
3.Standard Peptide
4.Assay Diluent(s)
5.Biotinylated Peptide
6.HRP-Streptavidin
7.TMB One-Step Substrate
8.Stop Solution
9.Assay Diagram
10.Positive Control Sample
11.Capture Antibody
12.Technical Manual

Other materials and equipment required:

The Assay Genie Human Neurokinin-B ELISA Kit will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • Precision pipettes to deliver 2 ul to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Orbital shaker
  • Aluminum foil
  • Saran Wrap
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Kit Protocol:

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