Human Neurokinin-A PharmaGenie ELISA Kit (SBRS0091)

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SKU:
SBRS0091
Product Type:
ELISA Kit
Reactivity:
Human
Applications:
ELISA
ELISA Type:
Competitive

Description

Human Neurokinin-A PharmaGenie ELISA - Kit Information

Neurokinin A, aka substance K, is a peptide hormone belonging to the tachykinin family, which also includes Substance P and Neurokinin B. All the peptides from tachykinin families are derived from two preprotachykinin genes - the PPT-A gene and PPT-B gene. The former encodes the sequences of Substance P. Neurokinin A. and Neuropeptide K and the latter encodes the sequence of Neurokinin B. Neuropeptide K and Neuropeptide gamma are N-terminally longer versions of neurokinin A. Neurokinin A has played a variety of roles in human. For example, it is reported that Neurokinin A may mediate bronchoconstriction through the non-adrenergic non-cholinergic nervous system in rat. There has been no clinical application reported for neurokinin A.

Human Neurokinin-A PharmaGenie ELISA - Kit Data

Product SKU:SBRS0091
Size:96T
Application:Cross Reactivity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Ghrelin, Nesfatin, Angiotensin II, NPY and APC.
Uniprot:P20366
Gene ID:6863
Gene Names:NKA / NKNA / TAC1 / TAC2
Synonyms:Protachykinin-1 (PPT) [Cleaved into: Substance P Neurokinin A (NKA) (Neuromedin L) (Substance K) Neuropeptide K (NPK) Neuropeptide gamma C-terminal-flanking peptide]
Target Species:Human, Mouse, Rat
Compatible Sample Types:Cell Culture Supernatants, Serum
Design Principle:Competition-based
Method of Detection:Colorimetric
Quantitative/Semi-Quantitative:Quantitative
Range:0.1-1,000 pg/ml
Sensitivity:0.8 pg/ml
Recommended Dilution:Human: 2X, Mouse: 2X, Rat: 2X
Storage/Stability:Standard, Biotinylated Neurokinin A peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2 to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Kit Components:

1.Pre-Coated 96-well Strip Microplate
2.Wash Buffer
3.Standard Peptide
4.Assay Diluent(s)
5.Biotinylated Peptide
6.HRP-Streptavidin
7.TMB One-Step Substrate
8.Stop Solution
9.Assay Diagram
10.Positive Control Sample
11.Capture Antibody
12.Technical Manual

Other materials and equipment required:

The Assay Genie Human Neurokinin-A ELISA Kit will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • Precision pipettes to deliver 2 ul to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Orbital shaker
  • Aluminum foil
  • Saran Wrap
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Kit Protocol:

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