Human Neuro Discovery Array (30 targets) (SARB0058)

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SKU:
SARB0058

Description

system_update_altDatasheet
Product Sku:SARB0058
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:30
Gene Symbols:ADIPOQ, BDNF, CCL11, CCL17, CCL2, CCL24, CCL26, CCL4, CNTF, CRP|CSF2, CXCL8, FAS, GDNF, IFNG, IL10, IL18, IL1A, IL1B, IL4, IL6, LIF, MMP2, MMP3, NGF, S100B, TGFB1, TIMP1, TNF, VEGFA
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
Adiponectin (ACRP30)
  • Gene Symbol: ADIPOQ ACDC ACRP30 APM1 GBP28
  • Gene ID: 9370
  • Accession #: Q15848
BDNF
  • Gene Symbol: BDNF
  • Gene ID: 627
  • Accession #: P23560
beta-NGF
  • Gene Symbol: NGF NGFB
  • Gene ID: 4803
  • Accession #: P01138
CNTF
  • Gene Symbol: CNTF
  • Gene ID: 1270
  • Accession #: P26441
CRP (C-Reactive Protein)
  • Gene Symbol: CRP PTX1
  • Gene ID: 1401
  • Accession #: P02741
Eotaxin-1 (CCL11)
  • Gene Symbol: CCL11 SCYA11
  • Gene ID: 6356
  • Accession #: P51671
Eotaxin-2 (MPIF-2/CCL24)
  • Gene Symbol: CCL24 MPIF2 SCYA24
  • Gene ID: 6369
  • Accession #: O00175
Eotaxin-3 (CCL26)
  • Gene Symbol: CCL26 SCYA26 UNQ216/PRO242
  • Gene ID: 10344
  • Accession #: Q9Y258
Fas (TNFRSF6/Apo-1)
  • Gene Symbol: FASLG FASL TNFSF6
  • Gene ID: 355
  • Accession #: P25445
IL-4
  • Gene Symbol: IL4
  • Gene ID: 3565
  • Accession #: P05112
GDNF
  • Gene Symbol: GDNF
  • Gene ID: 2668
  • Accession #: P39905
GM-CSF
  • Gene Symbol: CSF2
  • Gene ID: 1437
  • Accession #: P04141
IFN-gamma
  • Gene Symbol: IFNG
  • Gene ID: 3458
  • Accession #: P01579
IL-10
  • Gene Symbol: IL10
  • Gene ID: 3586
  • Accession #: P22301
IL-18
  • Gene Symbol: IL18 IGIF IL1F4
  • Gene ID: 3606
  • Accession #: Q14116
IL-1 alpha (IL-1 F1)
  • Gene Symbol: IL1A
  • Gene ID: 3552
  • Accession #: P01583
IL-1 beta (IL-1 F2)
  • Gene Symbol: IL1B
  • Gene ID: 3553
  • Accession #: P01584
IL-6
  • Gene Symbol: IL6
  • Gene ID: 3569
  • Accession #: P05231
IL-8 (CXCL8)
  • Gene Symbol: CXCL8 IL8
  • Gene ID: 3576
  • Accession #: P10145
LIF
  • Gene Symbol: LIF HILDA
  • Gene ID: 3976
  • Accession #: P15018
MCP-1 (CCL2)
  • Gene Symbol: CCL2 SCYA2
  • Gene ID: 6347
  • Accession #: P13500
MIP-1 beta (CCL4)
  • Gene Symbol: N/A
  • Gene ID: 388372; 6351
  • Accession #: P13236
MMP-2
  • Gene Symbol: MMP2 CLG4A
  • Gene ID: 4313
  • Accession #: P08253
MMP-3
  • Gene Symbol: MMP3 STMY1
  • Gene ID: 4314
  • Accession #: P08254
S100 B
  • Gene Symbol: S100B
  • Gene ID: 6285
  • Accession #: P04271
TARC (CCL17)
  • Gene Symbol: CCL17 SCYA17 TARC
  • Gene ID: 6361
  • Accession #: Q92583
TGF beta 1
  • Gene Symbol: TGFB1 TGFB
  • Gene ID: 7040
  • Accession #: P01137
TIMP-1
  • Gene Symbol: TIMP1 CLGI TIMP
  • Gene ID: 7076
  • Accession #: P01033
TNF alpha
  • Gene Symbol: TNF TNFA TNFSF2
  • Gene ID: 7124
  • Accession #: P01375
VEGF-A
  • Gene Symbol: VEGFA VEGF
  • Gene ID: 7422
  • Accession #: P15692
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Human Neuro Discovery C2 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product Type:
Protein Array
Analytes:
Adiponectin (ACRP30)
Analytes:
BDNF
Analytes:
beta-NGF
Analytes:
CNTF
Analytes:
CRP (C-Reactive Protein)
Analytes:
Eotaxin-1 (CCL11)
Analytes:
Eotaxin-2 (MPIF-2/CCL24)
Analytes:
Eotaxin-3 (CCL26)
Analytes:
Fas (TNFRSF6/Apo-1)
Analytes:
IL-4
Analytes:
GDNF
Analytes:
GM-CSF
Analytes:
IFN-gamma
Analytes:
IL-10
Analytes:
IL-18
Analytes:
IL-1 alpha (IL-1 F1)
Analytes:
IL-1 beta (IL-1 F2)
Analytes:
IL-6
Analytes:
IL-8 (CXCL8)
Analytes:
LIF
Analytes:
MCP-1 (CCL2)
Analytes:
MIP-1 beta (CCL4)
Analytes:
MMP-2
Analytes:
MMP-3
Analytes:
S100 B
Analytes:
TARC (CCL17)
Analytes:
TGF beta 1
Analytes:
TIMP-1
Analytes:
TNF alpha
Analytes:
VEGF-A
Reactivity:
Human
Applications:
Multiplex Array
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