Human NELFE Recombinant Protein (RPPB4084)

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SKU:
RPPB4084
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P18615

Description

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Product Name:Human NELFE Recombinant Protein
Product Code:RPPB4084
Size:10µg
Species:Human
Target:NELFE
Synonyms:Negative elongation factor E, NELF-E, RNA-binding protein RD, NELFE, RD, RDBP, Negative Elongation Factor Complex Member E, D6S45, RDP.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The NELFE solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 50% glycerol and 5mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMLVIPPG LSEEEEALQK KFNKLKKKKK ALLALKKQSS SSTTSQGGVK RSLSEQPVMD TATATEQAKQ LVKSGAISAI KAETKNSGFK RSRTLEGKLK DPEKGPVPTF QPFQRSISAD DDLQESSRRP QRKSLYESFV SSSDRLRELG PDGEEAEGPG AGDGPPRSFD WGYEERSGAH SSASPPRSRS RDRSHERNRD RDRDRERDRD RDRDRDRERD RDRDRDRDRD RERDRDRERD RDRDREGPFR RSDSFPERRA PRKGNTLYVY GEDMTPTLLR GAFSPFGNII DLSMDPPRNC AFVTYEKMES ADQAVAELNG TQVESVQLKV NIARKQPMLD AATGKSVWGS LAVQNSPKGC HRDKRTQIVY SDDVYKENLV DGF

NELFE is a vital component of NELF complex which represses RNA polymerase II transcript elongation. NELFE is similar to nuclear RNA-binding proteins but does not bind RNA. NELFE contains a tract of alternating basic and acidic residues, mainly arginine and aspartic acid.

NELFE Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 403 amino acids (1-380) and having a molecular mass of 45.6 kDa.NELFE is fused to a 23 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:RDBP: Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Belongs to the RRM NELF-E family.
UniProt Protein Details:

Protein type:Transcription, coactivator/corepressor; Mitochondrial

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: nucleoplasm; mitochondrion; nucleus

Molecular Function:protein binding; RNA binding; nucleotide binding

Biological Process: transcription from RNA polymerase II promoter; viral reproduction; positive regulation of viral transcription; RNA elongation from RNA polymerase II promoter; gene expression

NCBI Summary:The protein encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. [provided by RefSeq, Jul 2008]
UniProt Code:P18615
NCBI GenInfo Identifier:1350554
NCBI Gene ID:7936
NCBI Accession:P18615.3
UniProt Related Accession:P18615
Molecular Weight:43kDa
NCBI Full Name:Negative elongation factor E
NCBI Synonym Full Names:negative elongation factor complex member E
NCBI Official Symbol:NELFE��
NCBI Official Synonym Symbols:RD; RDP; RDBP; D6S45; NELF-E��
NCBI Protein Information:negative elongation factor E
UniProt Protein Name:Negative elongation factor E
UniProt Synonym Protein Names:RNA-binding protein RD
UniProt Gene Name:NELFE��
UniProt Entry Name:NELFE_HUMAN
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