Human NEF3 Recombinant Protein (GST tag)
- SKU:
- RPES5389
- Product Type:
- Recombinant Protein
- Species:
- Human
Description
| Product Name: | Human NEF3 Recombinant Protein (GST tag) |
| Product Code: | RPES5389 |
| Size: | 20µg |
| Species: | Human |
| Expression Host: | E.coli |
| Synonyms: | Neurofilament medium polypeptide, NF-M, 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein, NEFM, NEF3, NFM |
| Mol Mass: | 64.16 kDa |
| AP Mol Mass: | 60 kDa |
| Tag: | N-GST |
| Purity: | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin Level: | Please contact us for more information. |
| Bio Activity: | Testing in progress |
| Sequence: | Ser 2-Ser 358 |
| Accession: | P07197 |
| Storage: | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation: | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution: | Please refer to the printed manual for detailed information. |
| Background: | Human NF-M (neurofilament-medium length) is a 145‑150 kDa phosphorylated glycoprotein that belongs to the type IV intermediate filament family. It is 915 amino acids in length and contains a 100 amino acid (aa) N-terminal globular region, a 320 aa alpha -helical rod, or central region, and a 500 aa globular tail. The rod region contains a series of heptad repeats that contribute to a coiled-coil interaction with an adjacent NF-M molecule, the first step in intermediate filament oligomerization. The C-terminus shows phosphorylation and O-linked glycosylation. Human NF-M aa 2‑358, which includes most of the rod region, shares ~98% aa sequence identity with corresponding region of mouse, rat and canine NF-M. |
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