NECAP2 belongs to the NECAP family. The WXXF motifs mediate binding of accessory proteins to the ear-domain of AP-1, GGAs and AP-2 through hydrophobic interactions. Adaptin ear-binding coat-associated protein 2 can interacts with AP1G1 and AP2A1 components of the adapter protein complex AP-1 and AP-2. It also interacts with the GAE domain proteins GGA1, GGA2 and GGA3.
Product Name:
Human NECAP2 Recombinant Protein (RPES2100)
Product Code:
RPES2100
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Adaptin ear-binding coat-associated protein 2, NECAP endocytosis associated protein 2, NECAP2
Accession:
Q9NVZ3
Sequence:
Met 1-Phe263
Fusion tag:
N,C-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Adaptin ear-binding coat-associated protein 2 is produced by our E.coli expression system and the target gene encoding Met1-Phe263 is expressed with a 6His tag at the N-terminus, 6His tag at the C-terminus.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
31.5 kDa
AP Mol Mass:
35 kDa
Formulation:
Supplied as a 0.2 µm filtered solution of 20mM PB?150mM NaCl,pH7.4.
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.