Enzymes Recombinant Proteins

Human NDUFB4 Recombinant Protein (RPPB2009)

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SKU:
RPPB2009
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
O95168
Research Area:
Enzymes

Description

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Product Name:Human NDUFB4 Recombinant Protein
Product Code:RPPB2009
Size:20µg
Species:Human
Target:NDUFB4
Synonyms:B15, CI-B15, Complex I-B15, NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4, NADH-ubiquinone oxidoreductase B15 subunit.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The NDUFB4 solution (1mg/ml) contains 20mM Tris-HCl buffer, (pH 8.0), 0.4M Urea And 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMSFPKYK PSSLRTLPET LDPAEYNISP ETRRAQAERL AIRAQLKREY LLQYNDPNRR GLIENPALLR WAYARTINVY PNFRPTPKNS

NADH Dehydrogenase 1 Beta Subcomplex 4 (NDUFB4) is a member of the complex I NDUFB4 subunit family. NDUFB4 is a non-catalytic subunit of the multisubunit NADH:ubiquinone oxidoreductase, which is the first enzyme complex in the mitochondrial electron transport chain (complex I). Mammalian complex I is comprised of 45 different subunits and transfers electrons from NADH to ubiquinone.

NDUFB4 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 110 amino acids (1-87) and having a molecular mass of 12.6kDa.NDUFB4 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:NDUFB4: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I NDUFB4 subunit family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Mitochondrial; Energy Metabolism - oxidative phosphorylation; EC 1.6.5.3; EC 1.6.99.3; Membrane protein, integral; Oxidoreductase

Chromosomal Location of Human Ortholog: 3q13.33

Cellular Component: mitochondrial inner membrane; mitochondrial respiratory chain complex I; mitochondrion; nuclear membrane; nucleoplasm

Biological Process: mitochondrial electron transport, NADH to ubiquinone; mitochondrial respiratory chain complex I assembly; response to oxidative stress

NCBI Summary:This gene encodes a non-catalytic subunit of the multisubunit NADH:ubiquinone oxidoreductase, the first enzyme complex in the mitochondrial electron transport chain (complex I). Mammalian complex I is composed of 45 different subunits and transfers electrons from NADH to ubiquinone. [provided by RefSeq, Dec 2009]
UniProt Code:O95168
NCBI GenInfo Identifier:6093473
NCBI Gene ID:4710
NCBI Accession:O95168.3
UniProt Secondary Accession:O95168,B2RUY3, B9EJC7,
UniProt Related Accession:O95168
Molecular Weight:14,026 Da
NCBI Full Name:NADH dehydrogenase
NCBI Synonym Full Names:NADH:ubiquinone oxidoreductase subunit B4
NCBI Official Symbol:NDUFB4��
NCBI Official Synonym Symbols:B15; CI-B15��
NCBI Protein Information:NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4
UniProt Protein Name:NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4
UniProt Synonym Protein Names:Complex I-B15; CI-B15; NADH-ubiquinone oxidoreductase B15 subunit
Protein Family:NADH dehydrogenase
UniProt Gene Name:NDUFB4��
UniProt Entry Name:NDUB4_HUMAN
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