Enzymes Recombinant Proteins

Human NDUFA5 Recombinant Protein (RPPB2005)

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SKU:
RPPB2005
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q16718
Research Area:
Enzymes

Description

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Product Name:Human NDUFA5 Recombinant Protein
Product Code:RPPB2005
Size:10µg
Species:Human
Target:NDUFA5
Synonyms:NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, Complex I subunit B13, Complex I-13kD-B, CI-13kD-B, NADH-ubiquinone oxidoreductase 13 kDa-B subunit, NDUFA5, B13, NUFM, UQOR13, CI-13kB.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The NDUFA5 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMAGVLKK TTGLVGLAVC NTPHERLRIL YTKILDVLEE IPKNAAYRKY TEQITNEKLA MVKAEPDVKK LEDQLQGGQL EEVILQAEHE LNLARKMREW KLWEPLVEEP PADQWKWPI

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 (NDUFA5) is a member of the complex I NDUFA5 subunit family. The human NDUFA5 gene codes for the B13 subunit of complex I of the respiratory chain that transfers electrons from NADH to ubiquinone. The NDUFA5 protein localizes to the inner mitochondrial membrane as part of the seven component-containing, water soluble 'iron-sulfur protein' (IP) fraction of complex I, even though its exact role is undetermined.

NDUFA5 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 139 amino acids (1-116) and having a molecular mass of 15.8kDa.NDUFA5 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:NDUFA5: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I NDUFA5 subunit family.
UniProt Protein Details:

Protein type:EC 1.6.5.3; Mitochondrial; Energy Metabolism - oxidative phosphorylation; Oxidoreductase; EC 1.6.99.3

Chromosomal Location of Human Ortholog: 7q31.33

Cellular Component: mitochondrial inner membrane; mitochondrial respiratory chain complex I

Molecular Function:NADH dehydrogenase (ubiquinone) activity

Biological Process: cellular metabolic process; mitochondrial electron transport, NADH to ubiquinone

NCBI Summary:This nuclear gene encodes a conserved protein that comprises the B13 subunit of complex I of the mitochondrial respiratory chain. The encoded protein localizes to the inner mitochondrial membrane, where it is thought to aid in the transfer of electrons from NADH to ubiquinone. Alternative splicing results in multiple transcript variants. There are numerous pseudogenes of this gene on chromosomes 1, 3, 6, 8, 9, 11, 12, and 16. [provided by RefSeq, Apr 2014]
UniProt Code:Q16718
NCBI GenInfo Identifier:2499316
NCBI Gene ID:4698
NCBI Accession:Q16718.3
UniProt Related Accession:Q16718
Molecular Weight:
NCBI Full Name:NADH dehydrogenase
NCBI Synonym Full Names:NADH:ubiquinone oxidoreductase subunit A5
NCBI Official Symbol:NDUFA5��
NCBI Official Synonym Symbols:B13; NUFM; UQOR13; CI-13kB; CI-13KD-B��
NCBI Protein Information:NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5
UniProt Protein Name:NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5
UniProt Synonym Protein Names:Complex I subunit B13; Complex I-13kD-B; CI-13kD-B; NADH-ubiquinone oxidoreductase 13 kDa-B subunit
UniProt Gene Name:NDUFA5��
UniProt Entry Name:NDUA5_HUMAN
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