Enzymes Recombinant Proteins

Human NDUFA2 Recombinant Protein (RPPB2003)

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RPPB2003

Description

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Product Name:Human NDUFA2 Recombinant Protein
Product Code:RPPB2003
Size:10µg
Species:Human
Target:NDUFA2
Synonyms:NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2, Complex I-B8, CI-B8, NADH-ubiquinone oxidoreductase B8 subunit, NDUFA2, CD14, CIB8.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The NDUFA2 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMAAAAAS RGVGAKLGLR EIRIHLCQRS PGSQGVRDFI EKRYVELKKA NPDLPILIRE CSDVQPKLWA RYAFGQETNV PLNNFSADQV TRALENVLSG KA

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 (NDUFA5) is a member of the complex I NDUFA5 subunit family. The human NDUFA5 gene codes for the B13 subunit of complex I of the respiratory chain that transfers electrons from NADH to ubiquinone. The NDUFA5 protein localizes to the inner mitochondrial membrane as part of the seven component-containing, water soluble 'iron-sulfur protein' (IP) fraction of complex I, even though its exact role is undetermined.

NDUFA2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 122 amino acids (1-99) and having a molecular mass of 13.3kDa.NDUFA2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:NDUFA2: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
UniProt Protein Details:

Protein type:Mitochondrial; EC 1.6.99.3; Oxidoreductase; EC 1.6.5.3; Energy Metabolism - oxidative phosphorylation

Chromosomal Location of Human Ortholog: 5q31.2

Cellular Component: mitochondrial inner membrane; mitochondrial membrane; mitochondrial respiratory chain complex I

Molecular Function:NADH dehydrogenase (ubiquinone) activity

Biological Process: cellular metabolic process; mitochondrial electron transport, NADH to ubiquinone; mitochondrial respiratory chain complex I assembly

Disease: Leigh Syndrome

NCBI Summary:The encoded protein is a subunit of the hydrophobic protein fraction of the NADH:ubiquinone oxidoreductase (complex 1), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane, and may be involved in regulating complex I activity or its assembly via assistance in redox processes. Mutations in this gene are associated with Leigh syndrome, an early-onset progressive neurodegenerative disorder. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
UniProt Code:O43678
NCBI GenInfo Identifier:297206774
NCBI Gene ID:4695
NCBI Accession:NP_001171941.1
UniProt Secondary Accession:O43678,Q6IAY8, D6RJD6,
UniProt Related Accession:O43678
Molecular Weight:8,491 Da
NCBI Full Name:NADH dehydrogenase
NCBI Synonym Full Names:NADH:ubiquinone oxidoreductase subunit A2
NCBI Official Symbol:NDUFA2��
NCBI Official Synonym Symbols:B8; CD14; CIB8��
NCBI Protein Information:NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2
UniProt Protein Name:NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2
UniProt Synonym Protein Names:Complex I-B8; CI-B8; NADH-ubiquinone oxidoreductase B8 subunit
Protein Family:NADH dehydrogenase
UniProt Gene Name:NDUFA2��
UniProt Entry Name:NDUA2_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
O43678
Research Area:
Enzymes
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