Human NDFIP1 Recombinant Protein (RPPB4074)

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SKU:
RPPB4074
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9BT67

Description

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Product Name:Human NDFIP1 Recombinant Protein
Product Code:RPPB4074
Size:20µg
Species:Human
Target:NDFIP1
Synonyms:N4WBP5, NEDD4 family-interacting protein 1, Breast cancer-associated protein SGA-1M, NEDD4 WW domain-binding protein 5, Putative MAPK-activating protein PM13, Putative NF-kappa-B-activating protein 164, Putative NFKB and MAPK-activating protein,NDFIP1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:The NDFIP1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol and 0.1M NaCl.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMALALAA LAAVEPACGS RYQQLQNEEE SGEPEQAAGD APPPYSSISA ESAAYFDYKD ESGFPKPPSY NVATTLPSYD EAERTKAEAT IPLVPGRDED FVGRDDFDDA DQLRIGNDG

Nedd4 Family Interacting Protein 1 (NDFIP1) is a part of a small group of evolutionarily conserved proteins having 3 ransmembrane domains. NDFIP1 is a possible target for ubiquitination by the Nedd4 family of proteins. NDFIP, which is a member of a family of integral Golgi membrane proteins, also modulates EGFR signaling through multiple pathways.

NDFIP1 Human Recombinant produced in E. coli is. a single polypeptide chain containing 139 amino acids (1-116) and having a molecular mass of 14.8kDa. NDFIP1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:NDFIP1: Activates HECT domain-containing E3 ubiquitin-protein ligases, including NEDD4 and ITCH, and consequently modulates the stability of their targets. As a result, controls many cellular processes. Prevents chronic T-helper cells-mediated inflammation by activating ITCH and thus controlling JUNB degradation. In cortical neurons, mediates the ubiquitination of SLC11A2/DMT1 by NEDD4L, leading to down-regulation of the divalent metal transporter and protection of the cells from cobalt and iron toxicity. Modulates EGFR signaling through multiple pathways. In particular, may regulate the ratio of AKT1-to-MAPK8 signaling in response to EGF, acting on AKT1 probably through PTEN destabilization and on MAPK8 through ITCH-dependent MAP2K4 inactivation. As a result, may control cell growth rate. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Adaptor/scaffold; Membrane protein, multi-pass; Membrane protein, integralChromosomal Location of Human Ortholog: 5q31.3Cellular Component: Golgi membrane; perinuclear region of cytoplasm; extracellular region; integral to membrane; endosome membrane; cell cortexMolecular Function: protein binding; signal transducer activity; WW domain bindingBiological Process: negative regulation of interleukin-4 production; positive regulation of I-kappaB kinase/NF-kappaB cascade; ubiquitin-dependent protein catabolic process via the multivesicular body pathway; cellular iron ion homeostasis; regulation of lymphocyte differentiation; regulation of myeloid leukocyte differentiation; signal transduction; negative regulation of isotype switching to IgE isotypes; negative regulation of T cell proliferation; negative regulation of T-helper 2 type immune response; positive regulation of protein ubiquitination; negative regulation of inflammatory response; positive regulation of protein catabolic process; negative regulation of protein transport; negative regulation of transporter activity; regulation of isotype switching to IgG isotypes
UniProt Protein Details:
NCBI Summary:The protein encoded by this gene belongs to a small group of evolutionarily conserved proteins with three transmembrane domains. It is a potential target for ubiquitination by the Nedd4 family of proteins. This protein is thought to be part of a family of integral Golgi membrane proteins. [provided by RefSeq, Jul 2008]
UniProt Code:Q9BT67
NCBI GenInfo Identifier:74733098
NCBI Gene ID:80762
NCBI Accession:Q9BT67.1
UniProt Secondary Accession:Q9BT67,Q658T8, Q8N2E3, Q8N2F9, B2RDB8, D3DQF0
UniProt Related Accession:Q9BT67
Molecular Weight:24,899 Da
NCBI Full Name:NEDD4 family-interacting protein 1
NCBI Synonym Full Names:Nedd4 family interacting protein 1
NCBI Official Symbol:NDFIP1��
NCBI Official Synonym Symbols:N4WBP5��
NCBI Protein Information:NEDD4 family-interacting protein 1; Nedd4 WW domain-binding protein 5; putative MAPK-activating protein PM13; breast cancer-associated protein SGA-1M; putative NFKB and MAPK-activating protein; putative NF-kappa-B-activating protein 164
UniProt Protein Name:NEDD4 family-interacting protein 1
UniProt Synonym Protein Names:Breast cancer-associated protein SGA-1M; NEDD4 WW domain-binding protein 5; Putative MAPK-activating protein PM13; Putative NF-kappa-B-activating protein 164; Putative NFKB and MAPK-activating protein
Protein Family:NEDD4 family-interacting protein
UniProt Gene Name:NDFIP1��
UniProt Entry Name:NFIP1_HUMAN
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