Human NDE1 Recombinant Protein (RPPB4072)

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SKU:
RPPB4072
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9NXR1

Description

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Product Name:Human NDE1 Recombinant Protein
Product Code:RPPB4072
Size:20µg
Species:Human
Target:NDE1
Synonyms:Nuclear distribution protein nudE homolog 1, NudE, NDE1, NUDE, LIS4, NUDE1, HOM-TES-87.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The NDE1 solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 20% glycerol, 5mM DTT and 0.2M NaCl.
Stability:NDE1 Human Recombinant although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.
Purity:Greater than 85.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MEDSGKTFSS EEEEANYWKD LAMTYKQRAE NTQEELREFQ EGSREYEAEL ETQLQQIETR NRDLLSENNR LRMELETIKE KFEVQHSEGY RQISALEDDL AQTKAIKDQL QKYIRELEQA NDDLERAKRA TIMSLEDFEQ RLNQAIERNA FLESELDEKE NLLESVQRLK DEARDLRQEL AVQQKQEKPR TPMPSSVEAE RTDTAVQATG SVPSTPIAHR GPSSSLNTPG SFRRGLDDST GGTPLTPAAR ISALNIVGDL LRKVGALESK LASCRNLVYD QSPNRTGGPA SGRSSKNRDG GERRPSSTSV PLGDKGLDTS CRWLSKSTTR SSSSC

NDE1 is a cytoplasmic protein which is a member of the nudE protein family. Phosphorylated during mitosis, NDE1 is critical for the formation and function of the mitotic spindle in M phase and functions to regulate the Dynein-mediated transport of kinetochore proteins, as well as centrosome duplication during interphase. NDE1 is believed to interact with NDEL1, LIS1 and Dynein IC1/2, cytosolic in a signaling pathway which regulates the formation of neurons and is fundamental to the development of the cerebral cortex.

NDE1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 355 amino acids (1-335 a.a.) and having a molecular mass of 39.9kDa (Molecular weight on SDS-PAGE will appear higher).NDE1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:NUDE1: a microtubule-associated protein required for centrosome duplication, and the formation and function of the mitotic spindle. Associated with the centrosome, kinetochore, and spindle. Specifically phosphorylated during M phase, inducing its association with mitotic spindles. Appears to participate in dynein-mediated transport of kinetochore proteins to spindle poles along microtubules. Interacts with gamma tubulin, dynactin, CEP110, dynein,TCP1. PAFAH1B1, PCM-1 and PCNT. Concentrates at the plus ends of microtubules coincident with kinetochores in metaphase and anaphase, and at the cleavage furrow during cytokinesis.Two alternatively spliced human isoforms have been described.
UniProt Protein Details:

Protein type:Microtubule-binding; Cell cycle regulation; Cytoskeletal

Chromosomal Location of Human Ortholog: 16p13.11

Cellular Component: kinetochore; centrosome; kinesin complex; microtubule; membrane; spindle pole centrosome; cytosol; cleavage furrow

Molecular Function:protein domain specific binding; identical protein binding; protein binding; microtubule binding

Biological Process: cell migration; organelle organization and biogenesis; establishment of chromosome localization; neuron migration; microtubule nucleation; chromosome segregation; mitotic centrosome separation; centrosome localization; establishment of mitotic spindle orientation; cell division; neuroblast proliferation; vesicle transport along microtubule; mitotic cell cycle; cerebral cortex development; G2/M transition of mitotic cell cycle; centrosome duplication

Disease: Lissencephaly 4; Microhydranencephaly

UniProt Code:Q9NXR1
NCBI GenInfo Identifier:108860813
NCBI Gene ID:
NCBI Accession:Q9NXR1.2
Molecular Weight:37kDa; 38kDa
NCBI Full Name:Nuclear distribution protein nudE homolog 1
UniProt Protein Name:Nuclear distribution protein nudE homolog 1
Protein Family:Nuclear distribution protein
UniProt Gene Name:NDE1��
UniProt Entry Name:NDE1_HUMAN
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